抑眩宁颗粒对小鼠和大鼠缺血性眩晕的治疗作用及其机制

目的：探讨抑眩宁颗粒对缺血性眩晕小鼠和大鼠的治疗作用，阐述其对小鼠和大鼠脑组织炎症损伤及氧化应激的影响。方法：分别采用机械眩晕和结扎双侧颈总动脉（CCA）的方法建立眩晕小鼠和大鼠模型。选择小鼠和大鼠各60只，随机分为正常对照组、模型组、低剂量抑眩宁颗粒组（2.25 g·kg^-1/1.50 g·kg^-1）、中剂量抑眩宁颗粒组（4.50 g·kg^-1/3.00 g·kg^-1）、高剂量抑眩宁颗粒组（9.00 g·kg^-1/6.00 g·kg^-1）和阳性对照组（1.50 g·kg^-1/1.00 mg·kg^-1盐酸氟桂利嗪胶囊），每组10只。正常对照组和模型组小鼠和大鼠灌胃给予10 mL·kg^-1生理盐水，其余各组小鼠和大鼠给予10 mL·kg^-1相应药物，每天1次，连续给药7 d。分别测定各组小鼠和大鼠跳台逃避时间，检测各组小鼠的进食量，比色法检测各组大鼠脑组织中超氧化物歧化酶（SOD）活性和丙二醛（MDA）水平，ELISA法检测各组大鼠脑组织中肿瘤坏死因子α（TNF-α）和白细胞介素1β（IL-1β）水平。结果：与模型组比较，各剂量抑眩宁颗粒组和阳性对照组小鼠和大鼠跳台逃避时间缩短（P<0.05或P<0.01），各组小鼠进食量增多；与模型组比较，各剂量抑眩宁颗粒组大鼠脑组织中SOD明显升高（P<0.05或P<0.01），各剂量抑眩宁颗粒组大鼠脑组织中MDA水平明显降低（P<0.01）。与模型组比较，中和高剂量抑眩宁颗粒组大鼠脑组织中TNF-α和IL-1β水平明显降低（P<0.05或P<0.01）。结论：抑眩宁颗粒可抑制缺血性眩晕大鼠脑组织中的炎症损伤，降低氧化应激反应，从而对缺血性眩晕发挥治疗作用。

Therapeutic effects of YiXuanNing Granule on ischemic vertigo of mice and rats and their mechanisms

Objective:To investigate the therapeutic effects of YiXuanNing Granule on the ischemic vertigo of the mice and rats, and to clarify its effects on the inflammation damage and oxidative stress in brain tissue of the mice and rats. Methods:The vertigo models of mice and rats were established by mechanical vertigo and ligation of bilateral common carotid arteries(CCA),respectively. A total of 60 mice and 60 rats were selected and randomly divided into normal control group, model group, low dose of YiXuanNing Granule group (2.25 g·kg^-1/1.50 g·kg^-1), middle dose of YiXuanNing Granule group (4.50 g·kg^-1/3.00 g·kg^-1), high dose of YiXuanNing Granule group (9.00 g·kg^-1/6.00 g·kg^-1) and positive control group (1.50 g·kg^-1/1.00 mg·kg ^-1 flunarizine hydrochloride capsule),and there were 10 animals in each group. The mice and the rats in control group and model group were intragastrically administered with 10 mL·kg^-1 normal saline, and the mice and the rats in the other groups were given 10 mL·kg^-1 corresponding drug once a day for 7 d. The escape time of jumping off the platform of the mice and the rats in various groups were determined;the food intake of the mice in various groups were detected; the activities of superoxide dismutase (SOD) and the levels of malondialdehyde (MDA) in brain tissue of the rats in various groups were detected by colorimetric method. The levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in brain tissue of the rats in various groups were detected by ELISA method. Results:Compared with model group, the escape time of jumping off the platform of the mice and the rats in different doses of YiXuanNing Granule groups and positive control group was shortened (P<0.05 or P<0.01),and the food intake of the mice in various groups was increased. Compared with model group, the SOD activities in brain tissue of the rats in different doses of YiXuanNing Granule groups were significantly increased (P<0.05 or P<0.01); the MDA levels in brain tissue of the rats in different doses of YiXuanNing Granule groups were significantly decreased (P<0.01);compared with model group, the levels of TNF-α and IL-1β in brain tissue of the rats and the mice in middle and high doses of YiXuanNing Granule groups were significantly decreased (P<0.05 or P<0.01). Conclusion:YiXuanNing Granule can inhibit the inflammatory damage in brain tissue of the rats with ischemic vertigo and reduce the oxidative stress, thereby,it can play a therapeutic role in ischemic vertigo.