miR200家族在乳腺癌患者血浆中表达水平的检测及其临床意义

目的：检测microRNA200（miR200）家族在乳腺癌患者和正常人血浆中表达水平，评价其对乳腺癌筛查、进展和预后评估的潜在价值。方法：收集82例乳腺癌患者（乳腺癌组）和30名健康女性（对照组）的血浆标本，提取血浆中的微小RNAs （miRNAs），逆转录后采用实时荧光定量PCR法检测2组受试对象血浆中miR200家族（miR200a、miR200b、miR200c、miR141和miR429）的表达水平；采用受试者工作特征（ROC）曲线评价血浆中miRNAs的诊断价值，分析乳腺癌患者血浆miRNAs表达水平与临床病理参数的关系；采用Cox风险比例模型进行生存分析。结果：乳腺癌组患者血浆中miR141表达水平低于对照组（P<0.01），ROC曲线下面积（AUC）为0.717（P<0.01）；miR200b表达水平低于对照组（P=0.006），AUC为0.685（P=0.003）；但miR200a、miR200c和miR429表达水平与对照组比较差异无统计学意义（P=0.268，P=0.075，P=0.872）。联用miR141和miR200b时，AUC为0.735，比单用miR141稍有提升。miR200家族的表达水平与乳腺癌临床病理特征和术后总生存期无关联（P>0.05）。结论：miR200b和miR141有可能作为乳腺癌诊断的分子生物学指标。

Detection of expression levels of miR200 family in plasma of breast cancer patients and its significance

Objective: To detect the expression levels of microRNA200 (miR200) family in the plasma of the breast cancer patients and the normal controls, and to evaluate their potential values in the screening, progression and prognosis evaluation of breast cancer.Methods: A total of 82 cases of plasma samples of the patients with breast cancer (breast cancer group) and 30 cases of healthy plasma samples (control group) were collected.The microRNAs(miRNAs) were extracted from the plasma samples, the expression levels of miR200 family (miR200a, miR200b, miR200c, miR141 and miR429) were quantified by using real-time PCR technique.The receiver-operating characteristic (ROC) curve was used to assess the diagnostic value of miRNAs, and the associations between the levels of miRNAs and the clinicopathological characteristics were analyzed.Cox proportional hazard mode was used for survival analysis.Results: Compared with control group, the expression level of miR141 in the plasma of the patients in breast cancer group was decreased(P<0.01),and the area under the ROC curve (AUC) was 0.717(P<0.01); the expression level of miR200b was also decreased (P=0.006), the AUC was 0.685 (P= 0.003); the expression levels of miR200a, miR200c and miR429 had no significant differences between two groups(P=0.268, P=0.075, P=0.872). The AUC of combination of miR200b and miR141 was 0.735(P<0.01), the efficacy was superior to that of miR141 used alone. There were no statistically significant associations between the expression levels of miR200 family and the clinicopathological characteristics of breast cancer and postoperative overall survival(P>0.05).Conclusion: MiR200b and miR141 are likely to become the molecular biological parameters for the diagnosis of breast cancer.