复方米蒿籽软膏剂治疗烧烫伤的药效学实验研究

目的通过抗炎、镇痛、抑菌、水肿抑制实验及组织学观察等实验,观察复方米蒿籽软膏剂对烧烫伤的治疗作用。方法分别设阴性对照组、阳性对照组、高剂量组和低剂量组,采用二甲苯致小鼠耳廓肿胀试验、热板法、琼脂平板扩散法和水肿抑制作用实验,进行复方米蒿籽软膏剂对小鼠Ⅱ度烫伤的药效学评价。结果复方米蒿籽软膏高低剂量均对二甲苯致小鼠耳廓肿胀有抑制作用,且低剂量组对肿胀的抑制程度接近于阳性对照组,具有显著抗炎作用(P=0.0023);该制剂高剂量组还具有缓解热板性疼痛的效应(P=0.0365);并具有较好的抑制小鼠烫伤性水肿的作用(P=0.0390);尚可抑制葡萄球菌、大肠埃希菌为代表的G+细菌和G-细菌生长的作用。组织病理切片观察,用该制剂7天,皮肤组织伴有成纤维细胞生成,深层结构汗腺、毛囊腺恢复,表皮层次结构日趋完整,对试验性烧烫伤的治疗作用显著。结论复方米蒿籽软膏具有显著治疗实验动物烧烫伤创面的作用。

Experimental Study on Pharmacodynamical of Compound Mihaozi Ointment in the Treatment of Burn and Scald

Objective To observe the effects of Compound Mihaozi ointment on burn and scald by using anti-inflammatory, analgesic,bacteriostasis, edema inhibition, and histological staining assays. Methods The mice were used in this study, and separated into 4 groups including negative control, positive control, high-dose, and low-dose group. The pharmacodynamics of Compound Mihaozi ointment on second degree scald in mice was evaluated by Xylene-induced auricular swelling test, hot plate method, agar plate diffusion method, and edema inhibition test. Results The high and low doses of Compound Mihaozi ointment inhibited auricular swelling induced by xylene in mice, and the low dosage of the ointment, with significant anti-inflammatory effect(P=0.0023), inhibited auricle swelling similar to the positive control group. The high dosage of ointment had the effect of relieving hot-plate pain(P=0.0365), and also had a better effect of inhibiting scalded edema in mice(P =0.0390). Moreover, the ointment inhibited the growth of Staphylococcus(G+bacteria) and Escherichia coli.(G-bacteria). Histologically, in the mouse skin where treated with the ointment for 7 days, the fibroblasts were formed,sweat glands and follicular glands in deep skin structure were restored, and epidermis layers were becoming more and more complete.These findings indicated that the ointment had a significant therapeutic effect on experimental burn and scald. Conclusion Compound Mihaozi ointment has significant therapeutic effect on burn and scald wounds in experimental mice.