2-甲氧基雌二醇对神经胶质瘤荷瘤小鼠的抑瘤作用研究

目的 探讨2-甲氧基雌二醇(2-ME)对神经胶质瘤U251细胞移植瘤的增殖抑制、凋亡及可能的分子机制。方法 构建胶质瘤裸鼠移植瘤模型,经2-ME治疗后分别记录移植瘤的重量、体积; 通过移植瘤HE、免疫组化、TUNEL染色分析2-ME对移植瘤的增殖抑制、凋亡及对Bcl-2、VEGF表达水平的影响; 检测2-ME对裸鼠肝肾功能的影响。结果 2-ME对肿瘤的体积和重量均有抑制作用; HE染色显示实验组出现不同程度的肿瘤细胞密度减低; 免疫组化染色显示,随着2-ME水平的升高,肿瘤组织内Bcl-2、VEGF的表达水平逐渐下降; TUNEL染色显示2-ME诱导裸鼠移植瘤组织细胞凋亡; 肝肾功能检测未见损害发生。结论 2-ME在体内能有效抑制胶质瘤移植瘤的生长、诱导其凋亡,其抗肿瘤作用可能与Bcl-2、VEGF表达水平有关,且无明显毒副作用。

Antitumor effect of 2-methoxyestradiol on glioma xenograft tumor in vivo

ObjectiveTo investigate the proliferation inhibition, apoptosis and possible molecular mechanism of 2-methoxyestradiol(2-ME)on glioma xenograft tumor.Methods The xenograft model of glioma in nude mice was constructed in vivo. After treatment with 2-ME, the weight and volume of the xenograft tumor were recorded. The effects of 2-ME on proliferation inhibition, apoptosis and expression of Bcl-2 and VEGF in xenograft tumors were analyzed by HE, immunohistochemistry and TUNEL staining experiments. Liver and kidney function test in nude mice.Results 2-ME inhibited the volume and weight of tumors; HE staining showed that the experimental group showed different degrees of tumor cell density reduction,the results of immunohistochemistry showed that the expression of Bcl-2 and VEGF decreased gradually with the increase of 2-ME concentration. TUNEL staining showed that 2-ME induced apoptosis of xenograft tumor cells in nude mice,no damage occurred in liver and kidney function tests.Conclusion 2-ME could effectively inhibit the proliferation and induced apoptosis of glioma xenografts, and its anti-tumor effect might be related to Bcl-2 and VEGF, and no obvious side effects.