Ethanol tolerance and enhanced calcium/calmodulin-dependent phosphorylation of synaptic membrane proteins |
| |
| Authors: | B Shanley J Gurd H Kalant |
| |
| Affiliation: | 1. Department of Biochemistry, University of Queensland, St. Lucia, Qld. 4067 Australia;2. Department of Biochemistry, University of Toronto, Toronto, Ont. M5S 1A8 Canada;3. Department of Pharmacology, University of Toronto, Toronto, Ont. M5S 1A8 Canada |
| |
| Abstract: | The chronic effects of ethanol on synaptic membrane proteins was studied in ethanol-tolerant rats. Synaptic plasma membranes and postsynaptic densities were prepared from homogenates of forebrain and incubated in vitro with [gamma-32P]adenosine triphosphate in the presence and absence of calcium and calmodulin. In ethanol-tolerant animals, enhanced phosphorylation of synaptic plasma membrane but not postsynaptic density proteins was demonstrated in the presence of calcium and calmodulin. These results suggest that the development of tolerance to ethanol may involve alteration in neuronal sensitivity to calcium. |
| |
| Keywords: | ethanol calcium calmodulin phosphorylation synaptic membrane protein |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
