Identification of major allergens of wildflower honey |
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Authors: | Yadzir Zailatul Hani Mohamad Misnan Rosmilah Abdullah Noormalin Arip Masita Murad Shahnaz |
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Institution: | Allergy and Immunology Research Centre, Institute for Medical Research, Kuala Lumpur, Malaysia. zailatul@imr.gov.my |
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Abstract: | The aim of this study was to identify the major allergens of wildflower honey in local patients with atopic disease. SDS-PAGE revealed ten protein bands of 25 to 110 kDa, with a heavy cluster in region of 40-75 kDa. Immunoblotting demonstrated seven IgE-binding bands of 39 to 110 kDa. The 60 kDa protein had the highest frequency of IgE-binding (100%) followed by 54 kDa protein (95%), thus identified as the major allergens of wildflowerhoney. Our findings indicate that the allergen extract used for diagnosis of honey allergy contains both the 54 kDa and 60 kDa proteins. |
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