mdm2和p53基因在急性白血病细胞中的表达及意义

目的：探讨人急性髓细胞性白血病（ａｃｕｔｅ ｍｙｅｌｏｉｄ ｌｅｕｋｅｍｉａ．ＡＭＬ）细胞ｍｄｍ２及ｐ５３基因蛋白表达水平，相互关系及与临床预后的关系。方法：用免疫组化方法测定ｍｄｍ２及ｐ５３蛋白表达，ＨＰＩＡＳ－１０００高清晰度病理图像细胞测定系统分析结果。结果：７５％的ＡＭＬ患者表达不同程度的ｍｄｍ２蛋白，且在各种分型中均有ｍｄｍ２蛋白过度表达者：１８．７５％的ＡＭＬ患者表达ｐ５３蛋白；在ＡＭ

Expressions of mdm2 and p53 gene in acute myeloid leukemia cells

Objective: To explore mdm2 and p53 gene expressions in human acute myeloid leukemia(AML) cells, relationship between each other and their predictive value of chemotherapeutic efficacy Methods: The mdm2 and p53 gene expressions were determined by immunohistochemical staining and analysed by using high resolution pathological image analysis system Results:75%of AML patients was detected mdm2 protein in virous degree,which occured in any subtype leukemia The expression rate of p53 protein was 18 75%in 32 AML patients Furthermore,mdm2 (+)but p53 (-)was accounted for 65 63% The complete remission (CR)rate in mdm2 (+) patients (29 17%) was significantly lower than that in mdm2 (-) patients (83 3%)(P<0 01) The patients had lowest CR rate if their AML cells were both positive for mdm2 and p53 expressions Conclusions:Most of AML patients express mdm2 gene and,mdm2 (+),p53 (-) is the main expression pattern in AML patients Different expression patterns of the two proteins are related to the clinical outcome