| 高效液相色谱法同时测定蛇床子中蛇床子素和异茴芹素的含量 |
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| 引用本文： | 黄彩虹,雷鹏,刘韶,李新中,陈宝进.高效液相色谱法同时测定蛇床子中蛇床子素和异茴芹素的含量[J].中南药学,2009,7(3):171-174. |
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| 作者姓名： | 黄彩虹 雷鹏 刘韶 李新中 陈宝进 |
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| 作者单位： | 1. 中南大学湘雅医院药剂科,长沙,410008
2. 中南大学药学院,长沙,410013 |
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| 摘 要： | 目的建立高效液相色谱法同时测定蛇床子中蛇床子素和异茴芹素的含量。方法Promosil C18色谱柱（4．6mm×250mm．5μm）；流动相为甲醇-0．1形磷酸（80：20）；流速为0．8mL·min^-1；检测波长为265nm;柱温为30℃。结果蛇床子素在0．2191～2．191μg线性关系良好，平均回收率为98．53％；异茴芹素在0．0126～0．126μg线性关系良好，平均回收率为99．23％。结论该方法简便、快速、准确、重复性好，可用于蛇床子中蛇床子素和异茴芹素的同时测定。
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| 关 键 词： | 高效液相色谱法 蛇床子 蛇床子素 异茴芹素 含量测定 |
Simultaneous determination of osthole and isopimpinellin in Fructus Cnidii by HPLC |
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| HUANG Cai-hong,LEI Peng,LIU Shao,LI Xin-zhong,CHEN Bao-jin.Simultaneous determination of osthole and isopimpinellin in Fructus Cnidii by HPLC[J].Central South Pharmacy,2009,7(3):171-174. |
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| Authors: | HUANG Cai-hong LEI Peng LIU Shao LI Xin-zhong CHEN Bao-jin |
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| Institution: | 1. Department of Pharmacy, Xiangya Hospital, Central South University, Changsha 410008; 2. School of Pharmaceutical Sciences, Central South University, Changsha 410013) |
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| Abstract: | Objective To establish an HPLC method for the simultaneous determination of osthole and isopimpinellin in FructusCnidii. Methods HPLC analysis was performed on a Pmmosil C18 （250mm×4.6mm, 5μ m）column Thecolumn temperature was 30 ℃. The mobile phase was a mixed liquid with MeOH - 0. 1% H3PO4 （80 ： 20） and the flow rate was 0. 8mL·min^-1. The detection wavelength was 265 nm. Results A good linearity of osthole was at 0. 2191-2. 191μg, and the average recovery was 98. 15%. A good linearity of isopimpinellin was at 0. 0126-0. 126μg, and the average recovery was 99.23%. Conclusion The method is simple, rapid, accurate and reproducible for the simultaneous determination of osthole and isopimpinellin in Fructus Cnidii. |
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| Keywords: | HPLC Fructus Cnidii osthole isopimpinellin determination |
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