翼状胬肉中核糖体S6蛋白磷酸化、细胞周期蛋白D1的表达及意义

目的　研究核糖体Ｓ６蛋白磷酸化（ｒｉｂｏｓｏｍａｌＳ６ｐｒｏｔｅｉｎｐｈｏｓｐｈｏｒｙｌａｔｉｏｎ，Ｐ-Ｓ６）和细胞周期蛋白Ｄ１（ＣｙｃｌｉｎＤ１）在翼状胬肉中的表达及相关性，探讨哺乳动物雷帕霉素靶蛋白复合物１（ｍａｍｍａｌｉａｎｔａｒｇｅｔｏｆｒａｐａｍｙｃｉｎｃｏｍｐｌｅｘ１，ｍＴＯＲＣ１）信号通路在翼状胬肉发病机制中的作用。方法　收集翼状胬肉组织３１例，正常结膜组织１７例，利用免疫组织化学和Ｗｅｓｔｅｒｎｂｌｏｔ进行Ｐ-Ｓ６和ＣｙｃｌｉｎＤ１的检测及比较。结果　Ｗｅｓｔｅｒｎｂｌｏｔ检测６例翼状胬肉组织中Ｐ-Ｓ６蛋白／Ｓ６蛋白表达（１．１９６±０．１０１）显著高于正常结膜组织（０．２９５±０．０５６），差异有统计学意义（Ｐ＜０．０５）。免疫组织化学染色结果显示:翼状胬肉中Ｐ-Ｓ６、ＣｙｃｌｉｎＤ１的阳性表达率均为１００％（２５／２５），正常结膜组织中Ｐ-Ｓ６阳性表达率为１８．２％（２／１１）、ＣｙｃｌｉｎＤ１阳性表达率为９．１％（１／１１），正常结膜组织与翼状胬肉组织中Ｐ-Ｓ６与ＣｙｃｌｉｎＤ１表达差异均有统计学意义（均为Ｐ＜０．０５）。翼状胬肉组织中Ｐ-Ｓ６与ＣｙｃｌｉｎＤ１的表达呈正相关（ｒ＝０．７５２，Ｐ＜０．０５）。结论　ｍＴＯＲＣ１信号通路在翼状胬肉发病过程中起重要作用，并可能通过调控ＣｙｃｌｉｎＤ１的表达来实现。

Expression and significance of P-S6 and CyclinDl in pterygium

Objective To investigate the expression and relationship between ribosomal S6 protein phosphorylation ( P-S6) and CyclinDI in the pterygium , and explore the role of mammalian target of rapamycin complex I ( mTORCI ) signaling pathway in the pathogenesis of pterygium. Methods Thirty-one pterygium specimens were obtained , and 17 normal conjunctival specimens were also collected. The expression of PS6 and CyclinDI were examined by immunohistochemistry and Western blot. Results Western blot showed that the P-S6 protein/S6 protein in 6 cases of pterygium speci- men (1. 196 + 0. 101 ) was higher than that of normal conjunctiva tissues ( 0. 295 + 0. 056) , the difference was statistical sigruficant (P < 0. 05 ). Immunohistochemistry showed that the positive rate of P-S6 was 100. 0% ( 25/25 ) in pterygium and 18. 2% ( 2/ II) in normal conjunctiva, and the positive rate of CyclinDI was 100. 0% ( 25/25 ) in pterygium and 9. 1% ( 1/11 ) in normal conjunctiva. there were significant differences in the expressions of P-S6 and CyclinDI between pterygium and normal conjunctiva ( all P < 0. 05 ) . There existed positive correlation between the expression of P-S6 and CyclinDI in the pterygium ( r = 0. 752. P < 0. 05 ). Conclrision The activation of mTORCI signaling pathway plays an important role in the pathogenesis of pterygium, which may be achieved by regulating the expression of CyclinDI.