龙胆泻肝汤对大鼠实验性自身免疫性葡萄膜炎的治疗作用

目的　探讨龙胆泻肝汤对大鼠实验性自身免疫性葡萄膜炎（ｅｘｐｅｒｉｍｅｎｔａｌａｕｔｏ-ｉｍｍｕｎｅｕｖｅｉｔｉｓ，ＥＡＵ）的治疗作用。方法　Ｌｅｗｉｓ大鼠随机分为对照组６只、ＥＡＵ组１８只和ＬＸＴ组１８只，光感受器间维生素Ａ结合蛋白免疫ＥＡＵ组和ＬＸＴ组大鼠。观察大鼠生理特征及眼部炎症变化，出现葡萄膜炎症时给予ＬＸＴ组大鼠龙胆泻肝汤灌胃；免疫后１２ｄ比较各组大鼠眼组织病理学差异；免疫后每隔４ｄ检测房水蛋白及血清中白蛋白、球蛋白变化。结果　成功制备了ＥＡＵ大鼠模型；免疫后５ｄ大鼠开始出现葡萄膜炎症状，１２ｄ炎症最重。ＥＡＵ大鼠肛温在１ｄ、７ｄ、１０ｄ和１２ｄ高于对照组（Ｐ＝０．０２０、０．０００、０.０１５、０．００１）；免疫后１２ｄ，ＥＡＵ组房水蛋白浓度为（３６．０３±３．２３）ｇ?Ｌ－１，ＬＸＴ组为（２４．６７±２．６０）ｇ?Ｌ－１，差异有显著统计学意义（Ｐ＝０．０００）；组织病理学显示ＥＡＵ组视网膜结构破坏程度明显高于ＬＸＴ组（Ｐ＝０．０００）。ＥＡＵ组免疫后４ｄ、８ｄ及１２ｄ白蛋白均低于对照组（Ｐ＝０．０４５、０．０００、０.０３３），ＬＸＴ组４ｄ和８ｄ白蛋白低于正常（Ｐ＝０.０４５、０．００５），但１２ｄ时恢复至正常；１２ｄ时ＥＡＵ组球蛋白明显高于正常（Ｐ＝０．０４７），ＬＸＴ组未见明显异常。结论　龙胆泻肝汤能有效减轻ＥＡＵ大鼠前房炎症，减少炎症细胞浸润，保护眼部组织结构，增强机体抗氧化能力，调节免疫状态，对ＥＡＵ发挥治疗作用。

Treatment efficacy of Longdan Xiegan Tang on rats with experimental autoimmune uveitis

Objective To study the treatment efficacy of Longdan Xiegan Tang on rats with experimental autoimmune uveitis ( EAU ) . Methods Lewis rats were randomly divided into control group (6 rats) , EAU group ( 18 rats) and LXT group ( 18 rats) . Rats in EAU and LXT groups were immunized with interphotoreceptor retinoid-binding protein, and then the changes of physiological features were recorded during the observational duration. Further . rats in LXT group were treated with Longdan Xiegan Tang when inflammation symptom was observed in eyes. The changes of histopathology were compared on day 12 post immunization , and aqueous humor protein , albumin and globulin in serum were detected every 4 days. Results The model of uveitis with burrung-heat in the liver and gallbladder type has successfully been constructed. Uveitis inflammation was observed on day 5 and the severity peaked on day 12 post immunization. The rectal temperature of EAU rats was higher than those of normal ones on day I , day 7 , day 10 and day 12 post immunization(P =0. 020 .0. 000 . 0. 015 , 0. 001 ). On day 12, the concentrations of aqueous humor protein were ( 36. 03 + 3. 23 ) g . L -l in EAU group and ( 24. 67 + 2. 60) g . L-1 in LXT group , respectively, and there was statistically significant differences (P = 0. 000 ) . Meanwhile . the morphologic changes of organizational structure of retina also showed sigruficant differences between EAU group and LXT group on day 12 (P = 0. 000) . Moreover, the levels of albumin in EAU group on day 4, day 8 and day 12 post immunization were lower than that in the control group (P = 0. 045 . 0. 000 . 0. 033 ) , the levels of albumin in LXT group on day 4 and day 8 post immunization were lower than that in the control group (P =0. 045 , 0. 005 ) , and returned to the normal at day 12; The level of globulin in EAU ~oup on day 12 post immuruzation was higher than that in the control group (P = 0. 047) , while no difference was found in LXT group. Conclrision Longdan Xiegan Tang can efficiently alleviate anterior chamber inflammation and reduce inflammatory cell infiltration of EAU rats , protect the organization structure of eyes , enhance the antioxidant capacity, restore immune state , and has an ideal efficacy on uveitis.