牵拉培养对表皮细胞生长及EGF分泌的影响

目的：研究牵拉培养对表皮细胞生长的影响以及内源性EGF的分泌情况。方法：用酶消化法对表皮细胞进行原代培养，将实验分为二组：实验组和对照组，实验组采用细胞负压牵引装置培养，对照组细胞不受牵拉，连续观察72小时，分别于12、24、36、48、60、72小时，收集细胞培养液，同时于72小时观察表皮细胞在牵拉条件下和非牵拉条件下的生长情况：用ELISA法测定表皮细胞在牵拉条件下和非牵拉条件下分泌EGF的情况。结果：实验组表皮细胞经72小时牵拉后生长良好，而对照组的细胞死亡，实验组牵拉36小时EGF浓度为16．03μg／mL，其他时间点未测到，而非牵拉组各时间点均未测出EGF。结论：牵拉不但可促进表皮细胞的增殖和生长，而且可促进表皮细胞分泌EGF．

Effect of strain on the growth and the secretion of EGF in human epidermal cells in vitro

Objective To explore the effect of strain on the growth and secretion of EGF of human epidermal cells in vitro. Methods the study was performed with a vitro model of expansion in which epidermal cells were cultured on a flexible-bottom membrane that could be mechanically deformed by vacuum.Epidermal cells were respectively seeded equally on flexible membranes coated with type I collagen.The effect of cyclic strain on human epidermal cells in vitro was investigated.Cells were incubated for 72hr which were cultured in serum-free medium.The medium were collected respectively after 12-72 h of stretch.EGF concentrations were determined using an enzyme-linked immunosorbent assay. Results After 72h of stretch epidermal grew well.Conversely epidermal cells in the control group had died after 72h.EGF after 36h increased significantly,reaching 16.03pg/ml after 36h,and could not determined apart from 36h after stretch.Conversely,EGF could not determined during 12-72h in control group. Conclusion These findings suggest that cyclic strain promotes proliferation and secretion of EGF of cultivated epidermal cells.