血小板保存期间血小板计数和P-选择蛋白的变化

探讨CS 30 0 0plus血细胞分离机全密闭 5天保存袋保存的血小板数量和质量的变化 ,研究在 (2 2± 2 )℃条件下振荡和静置两种方法保存的血小板有无差异。采用SysmexF 82 0型全自动血细胞分析仪对机采血小板产品计数 ,应用酶联免疫吸附法 (ELISA)定量检测机采血小板上清液中P 选择蛋白的含量。结果显示 ,振荡与静置两种方法保存的血小板产品中血小板计数和P 选择蛋白含量均无显著性差异。随保存时间延长 ,振荡和静置保存的血小板计数逐渐减少和P 选择蛋白逐渐增多 ,其中两组内的血小板计数在保存 0 - 72小时以内均无显著性差异 ;而在保存 96 - 1 2 0小时后与保存前比较都有显著性差异。振荡保存组 0 - 72小时内 ,每相邻 1天之间P 选择蛋白量无显著性差异 ,其余各时间段均有显著性差异 ;静置保存组 0 - 72小时内各时间段均无显著性差异。结论 :①使用CS 30 0 0plus血细胞分离机全密闭 5天保存袋 ,对血小板制品的有效保存期以 3天为宜 ;②在 (2 2± 2 )℃条件下 ,振荡保存的血小板在数量和质量上并不优于静置保存的血小板。

Change of Platelet Count and P-Selectin in Preserved Platelet Concentrates

To observe the change of quantity and quality of platelets preserved in a full-sealed bag, and explore the difference of platelets preserved in oscillating and static conditions at (22 +/- 2) degrees C, the platelet concentrates were prepared with a CS-3000-plus blood cell separator, the platelet counts were performed with automatic blood cell analyzer and P-selectin in supernatant of platelet concentrates was detected by ELISA. The results showed that both of platelet count and P-selectin content in the platelet concentrates had no significant difference between oscillating and static preservation condition. With prolongation of preserved time, the platelet count decreased and P-selectin content increased gradually in both preserved conditions. There was no difference in the platelet counts during 0 - 72 hours preservation in both conditions, and significant difference was seen in 96 - 120 hours preservation. It was concluded that the expired date for platelet product preserved in CS-3000-plus blood cell separator full-sealed system should be 3 days. Under the condition of (22 +/- 2) degrees C, the quality of the platelet preserved in oscillating state is not superior to static preservation.