丝素蛋白/左旋聚乳酸支架材料与软骨细胞体外细胞相容性研究

目的:观察静电纺丝支架材料丝素蛋白/左旋聚乳酸(SF/PLLA)的体外细胞相容性,探索其作为软骨组织工程支架材料的可行性,为进一步的体内及动物实验奠定基础。方法:将兔膝关节软骨细胞与丝素蛋白/左旋聚乳酸(SF/PLLA)支架材料复合培养,在第3、7、14天分别作HE染色和阿利新蓝+核固红染色,扫描电镜检验细胞黏着情况,MTT试验检测细胞在支架上的增殖情况。结果:细胞在支架上可以获得良好的粘附,细胞增殖良好,无细胞表型的变化。结论:丝素蛋白/左旋聚乳酸(SF/PLLA)具有良好的细胞相容性,可作为软骨组织工程支架材料。

Cytocompatibility Study of Silk Fibroin/poly-L-lactic Acid Scaffold and Chondrocytes in Vitro.

Objective: To lay the foundation for further body and animal experiment, we observe the cytocompatibility on electrostatic spinning scaffold of silk fibroin/poly-L-lactic acid (SF/PLLA) in vitro and explore the feasibility in cartilage tissue engineering scaffolds. Methods: The rabbit knee joint chondrocyte were co-cultured with silk fibroin/poly-L-lactic acid (SF/PLLA) scaffold. Hematoxylin-eosin staining and Alcian blue- nuclear fast red staining were tested on day 3, 7 and 14 respectively. Cell adhesion was tested by scanning electron microscopy (SEM). Cell proliferation was determined by MTT test. Results: Adhesion, proliferation, and growth of chondrocyte derived from rabbit knee joint chondrocytes were great on the surface of silk fibroin/poly-L-lactic acid (SF/PLLA). The chondrocytes cultured in vitro maintained the specific chondrocytes phenotype on the scaffold. Conclusion: Silk fibroin/poly-L-lactic acid (SF/PLLA) scaffold exhibits excellent cytocompatibility, and can be used for cartilage tissue engineering.