血竭素高氯酸盐对舌鳞癌细胞Tca-8113增殖及凋亡的影响

目的:研究血竭素高氯酸盐(Dracorhodin perchlorate,DP)对舌鳞癌细胞Tca-8113增殖和凋亡的影响并初步探讨其相关机制。方法:以舌鳞癌细胞Tca-8113为研究对象,采用CCK-8、流式细胞术分析及Western Blot等方法检测不同浓度DP对Tca-8113细胞的增殖、凋亡及核因子E2-相关因子2(nuclear factor E2-related factor 2,Nrf2)、血红素氧合酶 1(heme oxygenase 1,HO-1)蛋白表达的变化。采用SPSS17.0统计软件对实验结果进行分析。结果:CCK-8检测表明,与空白对照组相比,DP可以显著抑制细胞增殖,且对细胞的增殖抑制作用有时间-剂量依赖性(P<0.05)。流式细胞术及Western Blot检测结果显示DP可以促进细胞凋亡(P<0.05),并提高Nrf2、HO-1在Tca-8113细胞中的表达。结论:DP能抑制舌鳞癌细胞Tca-8113增殖并诱导其发生凋亡,其作用可能与Nrf2/HO-1信号通路激活有关;可能成为抗口腔癌药物。

Effect of Dracorhodin Perchlorate on the Proliferation and Apoptosis of Tongue Squamous Carcinoma Cell Line Tca-8113.

Objective: To investigate the effect of dracorhodin perchlorate (DP) on the cell proliferation and apoptosis and to discuss relevant signaling pathways in human tongue squamous carcinoma cell line Tca-8113 . Methods: The Tca8113 cells were used as research object in this experiment. The growth, apoptosis and the expression of nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase 1(HO-1) were detected in human tongue carcinoma cell line(Tca-8113), using cell counting kit-8 (CCK-8), the flow cytometry and Western blot to assay. All statistical analysis was performed using the SPSS 17.0 statistical package. Results: CCK-8 assay proved that there was a much pronounced decrease in cell viability on treatment with DP compared with control group and the time- and dose-dependent effects of treatment with DP on the growth of cells (P<0.05). Flow cytometric analysis and Western blot demonstrated that DP promoted cell apoptosis (P<0.05) and elevated the expression of Nrf2 and HO-1 proteins in Tca-8113 cells. Conclusion: DP inhibited tongue squamous carcinoma Tca-8113 cells growth and induced apoptosis, which may be relevant with the activation of Nrf2/HO-1signaling pathway in oral cancer cells. DP could be developed as an agent against OSCC.