甲氧基聚乙二醇修饰改造RhD(+)血型的初步研究

Rh血型与ABO血型一样是重要的血型系统，Rh血型不符会引起严重的输血反应及新生儿溶血病。中国人群中RhD阴性血型的比例仅为0．2％-0．5％，将RhD阳性红细胞改造成RhD阴性红细胞在临床输血中有重要的意义。本研究用4种不同端基的甲氧基聚乙二醇(mPEG)，修饰A型、B型、AB型和O型的RhD( )红细胞，比较4种mPEG衍生物对RhD抗原的修饰效果。同时观察mPEG修饰对红细胞形态、结构和功能的影响。结果表明，mPEG—BTC(苯并三唑端基PEG)较其他3种PEG的修饰效果好，在1mmol／L时可以有效地遮蔽红细胞表面RhD抗原，而对红细胞形态、渗透脆性、自身溶血、乙酰胆碱酯酶、胆固醇、ATP、2，3-DPG含量以及变形性无影响。结论：初步实现了将RhD( )红细胞修饰改造成RhD(-)红细胞的目的。

Preliminary Study on Conversion of RhD Positive Red Blood Cells to RhD Negative by Modification with Methoxy Polyethylene Glycol

Rh is a very important blood group like ABO blood system in transfusion medicine. It causes severe transfusion reaction and hemolytic disease of the newborn (HDN) if RhD blood group does not match between the donor and the recipient. The population of RhD negative is only about 0.2%-0.5% in Chinese. Conversion of RhD positive RBCs to RhD negative is very important in clinical transfusion. This study was to try to modify RhD antigen located on the surface of A, B,O and AB red blood cells in order to convert RhD positive to RhD negative by the modification of four kinds of methoxypolyethylene glycol (mPEG) derivatives and to observe the effect of mPEG modification on cell morphology, structure and function. The result demonstrated that modification efficiency of mPEG BTC (mPEG benzotriazole carbonate) was better than other three kinds of mPEG derivatives. It could camouflage RhD antigen efficiently when the concentration reached to 1 mmol/L. The result also showed that there were no harmful effects of mPEG modification on cell morphology, osmotic fragility, hemolysis, AchE, cholesterol, ATP, 2,3 DPG and deformability. It is suggested that success in converting RhD positive RBCs to RhD negative was preliminarily achieved.