建立显微分离细胞技术并检测胃癌细胞内p53基因突变

目的 获取较纯的微量胃癌细胞并检测ｐ５３基因的突变。方法 在显微镜下，直接分离出胃癌细胞中微小癌灶内细胞，并应用单链构象多态（ＳＳＣＰ）技术分析该微量细胞中ｐ５３基因的突变。结果 在１７１例胃癌组织的石蜡标本中，有７０例获得可靠结果，包括６例早期胃癌和６４例进展期胃癌，该方法总成功率为４１．０％。结论 显微分离细胞技术十分有利于观察癌前病变、原位癌及浸润的基因变化；该方法直接证明早期胃癌细胞中ｐ５

A microdissection technique for the molecular diagnosis of p53 gene mutations in gastric carcinoma

Objective: To obtain pure gastric carcinoma cells for the molecular diagnosis of p53 gene mutations in gastric carcinoma. Methods: A microdissection technique was developed which consisted in picking out minute cell populations from paraffinembedded tissue sections under light microscopy and analyzing p53 gene mutations in the minute carcinoma cell populations by SSCP. Results: Of 171 cases of gastric carcinoma, 70 were analyzed successfully with a successful rate of 41.0%. including 6 cases of early gastric carcinoma (EGC) and 64 cases of advanced gastric carcinoma (AGC). Conclusions: The microdissection technique proposed proved to be useful in gene studies of premalignant. in situ as well as invasive cancerous lesions and the method could directely demonstrate p53 gene mutation in EGC.