| PAI—1基因4G／5G多态性与2型糖尿病合并肾病的相关性研究 |
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| 引用本文： | 李长贵,董砚虎,王海燕,司元国,吕文山,翟木绪,王军,柳林.PAI—1基因4G／5G多态性与2型糖尿病合并肾病的相关性研究[J].中华糖尿病杂志,2001,9(6):333-336,340. |
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| 作者姓名： | 李长贵 董砚虎 王海燕 司元国 吕文山 翟木绪 王军 柳林 |
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| 作者单位： | 李长贵（青岛市内分泌糖尿病医院青岛市海慈医疗集团,266033）
董砚虎（青岛市内分泌糖尿病医院青岛市海慈医疗集团,266033）
王海燕（青岛市内分泌糖尿病医院青岛市海慈医疗集团,266033）
司元国（青岛市内分泌糖尿病医院青岛市海慈医疗集团,266033）
吕文山（青岛市内分泌糖尿病医院青岛市海慈医疗集团,266033）
翟木绪（青岛市内分泌糖尿病医院青岛市海慈医疗集团,266033）
王军（青岛市内分泌糖尿病医院青岛市海慈医疗集团,266033）
柳林（青岛市内分泌糖尿病医院青岛市海慈医疗集团,266033） |
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| 摘 要： | 目的探讨血浆纤溶酶原激活物抑制物-1(PAI-1)基因启动子区4G/5G多态性与中国北方汉族人2型糖尿病合并肾病的相关性.方法运用等位基因特异性引物PCR扩增技术,检测病程超过10年的2型糖尿病患者(1 43例)和健康人(85例)的PAI-1基因4G/5G多态位点的基因型,用发色底物法测血浆PAI-1活性.结果 (1)糖尿病肾病组血浆PAI-1活性明显高于糖尿病非肾病组(P＜0.05).(2)4G/4G基因型者PAI-1活性明显高于4G/5G和5G/5G者(P＜0.005).(3)糖尿病肾病组PAI-1 4G/4G基因型频率(39%)和4G等位基因频率(60.5%)高于糖尿病非肾病组(33%和56.5%),但无统计学意义(P＞0.05).结论 PAI-1活性升高是糖尿病肾病的独立危险因素,PAI-1基因启动子区4G/5G多态性与PAI-1活性密切相关,4G/4G基因型可能是易发糖尿病肾病的遗传标记.
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| 关 键 词： | 血浆纤溶酶原激活物抑制物－1 基因多态性 糖尿病肾病 |
Association of plasminogen activator inhibitor type-1 4G/5G polymorphism and type 2 diabetes with nephropathy |
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| Li Chang Gui,Dong Yanhu,Wang Haiyan,et al.Association of plasminogen activator inhibitor type-1 4G/5G polymorphism and type 2 diabetes with nephropathy[J].Chinese Journal of Diabetes,2001,9(6):333-336,340. |
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| Authors: | Li Chang Gui Dong Yanhu Wang Haiyan |
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| Abstract: | Objective To investigate the association of the PAI 1 promoter 4G/5G polymorphism and diabetic nephropathy in type 2 diabetes mellitus of North China.Methods The 4G/5G allele polymorphism in the PAI 1 gene promoter region were genetyped by allele specific PCR in 143 type 2 diabetic patients who had diabetes for over 10 years and 85 normal controls.The activity of PAI 1 was assayed by a chromogenic substrate method.Results Patients with type 2 diabetic nephropathy had a higher frequency of PAI 1 4G/4G genotype (39%) and 4G allele (60.5%) than those without nephropathy (33% and 56.5%),though not significantly ( P >0.05).The plasma PAI 1 activity in the diabeties with nephropathy was higher than that in the subjects without nephropathy ( P <0.05).PAI 1 activity was significantly higher in 4G allele homozygous than those in 4G/5G heterozygous and 5G homozygous ( P <0.005).Conclusion Elevated plasma PAI 1 activity is a risk factor for diabetic nephropathy.The presence of the 4G allele of PAI 1 gene was associated with a higher level of PAI 1 activity,and may involved in the generation of diabetic nephropathy. |
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| Keywords: | Plasminogen activator inhibitor 1 Gene polymorphism Diabetic nephropathy |
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