归芍安宫胶囊对子宫内膜异位症大鼠细胞因子的影响

目的：研究归芍安宫胶囊（GSAGC）对子宫内膜异位症（EMS）大鼠细胞因子的影响，初步探讨GSAGC对EMS的作用机理。方法：EMS模型大鼠随机分为GSAGC高剂量组、GSAGC中剂量组、GSAGC低剂量组、达那唑组及模型组，另设假手术组为对照。四周后观察GSAGC对大鼠异位子宫内膜体积、抑制率及对大鼠异位子宫内膜形态学的变化的影响；并用放免法检测GSAGC对血清白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）的含量的影响；用免疫组化方法检测GSAGC对异位子宫内膜血管内皮生长因子（VEGF）表达的影响。结果：GSAGC治疗四周后，和模型组比较，GSAGC高、中、低剂量组大鼠的异位子宫内膜体积明显缩小（P〈0．01，P〈0．05），抑制率明显升高（P〈0．05），并且IL-6、TNF—α的含量也较模型组有明显降低（P〈0．01，P〈0．05），同时GSAG也明显减少了模型组大鼠异位子宫内膜VEGF的表达（P〈0．01，P〈0．05）。结论：GSAGC对大鼠EMS具有一定的治疗作用，其作用机理可能是通过减少IL-6、TNF-α等免疫因子的生成，使VEGF的生成减少，异位内膜的血管形成被阻断，抑制病灶的形成和生长。

Effects on Cell Factor in Endometriosis Rats of GuiShaoAnGong Capsule

Objective： To study the effects on cell factor in endometriosis rats of GuiShaoAnGong Capsule （GSAGC）, and evaluate the mechanism of it preliminarily. Methods： EMS model rats were randomly divided into high dose GSAGC treated group, middie dose GSAGC treated group, low dose GSAGC treated group, Danazol treated group and model group. Another, sham operation group were acted as control group. The volume of ectopic endometrium, inhibitory rate and pathologic changes of ectopic endometrium in rats of GSAGC were observed. The effects on the levels of Interleukin-6 （IL-6） and Tumor Necrosis Factor alpha （TNF-α） in the blood serum were detected by radioimmunoassay （RIA） and the expressions of Vascular Endothelial Growth Factor （VEGF） were detected by immunhistochemistry （IHC）. Results： Compared with model group, volumes of ectopic endometrium of GSAGC treated groups deflated after four weeks treated （P 〈 0. 01, P 〈 0. 05）, and inhibitory rates advanced （P 〈 0. 05 ）, and the levels of IL - 6 and TNF -α decreased （ P 〈 0. 01, P 〈 0. 05 ）, and the expression of VEGF diminished obviously （ P 〈 0. 01, P 〈 O. 05 ） Conclusion ： GSAGC shows certain treatment on endometriosis of rats. It is possible for the mechanisms that GSAGC could decrease the form of IL - 6, TNF - α and VEGF to inhibite the form and growth of ectopic endometrium.