解偶联蛋白3基因多态性与中国人2型糖尿病的相关性研究

目的 探讨解偶联蛋白３（ＵＣＰ３）基因二核苷酸（ＣＡ）ｎ患联重复序列多态标记与中国汉族人２型糖尿病的关系。方法 用ＰＣＲ－变性聚丙烯酰胺凝胶电泳及银染方法，检测２０８例南京地区汉族人（２型糖尿病１０６例，正常对照１０２例）ＵＣＰ３基因的（ＣＡ）ｎ串联重复序列多态标记比较各组间的等位基因频率和基因型频率。结果 ２型糖尿和正常对照组因等位基因频率的分布存在显著性差异（Ｘ＾２＝２６．１２，Ｐ〈０．００５

Association Study between Polymorphism of Uncoupling Protein 3 Gene and Type 2 Diabetes Mellitus in Chinese

Objective To investigate the association between dinucleotide (CA)n tandem repeat polymorphism of uncoupling protein 3 gene and type 2 diabetes mellitus in Chinese. Methods A casecontrol study on 208 Chinese subjects from Nanjing region (106 patients with type 2 diabetes and 102 normal subjects) was performed. The polymorphism of (CA)n tandem repeat of UCP 3 gene was determined by PCRdenaturing polyacrylamide gel electrophoresis and silver staining. Results The prevalence of UCP 3 gene allele frequency in type 2 diabetic patients was significantly different from that in control subjects. An elevated frequency of 6,7,8 allele was observed in type 2 diabetes mellitus as compared with control subjects. Significant differences of UCP 3 gene allele frequency were found between the Chinese and the Caucasian population. Conclusion Dinucleotide (CA)n tandem repeat polymorphism of uncoupling protein 3 gene is associated with type 2 diabetes mellitus in Chinese.