塞来昔布增强博来霉素对人舌鳞状细胞癌Tca8113杀伤作用的研究

目的 研究环氧合酶2(cyclooxygenase-2,COX-2)抑制剂塞来昔布(celecoxib)增强博来霉素(bleomycin)对人舌鳞状细胞癌Tca8113细胞的杀伤作用.方法 用含不同浓度的塞来昔布和博来霉素培养液培养Tca8113细胞24、48、72 h后,甲基噻唑基四唑(MTT)法检测细胞生长抑制率,并采用金正钧法判断药物合用的相互作用;流式细胞术检测Tca8113细胞周期进程和诱导细胞凋亡的作用.结果 小剂量的塞来昔布(10 μmol/L)可增强博来霉素对Tca8113细胞的生长抑制作用,增强博来霉素阻滞Tca8113细胞周期进程的作用,其阻滞G1期细胞进入S期的作用最为明显,导致G0、G1细胞的数量增加(60.93±0.32)%],S期(30.57±0.78)%]、G2及M期(8.50±0.50)%]细胞减少,同时,细胞凋亡率(1.87±0.11)%]显著提高.结论 小剂量的塞来昔布可增强博来霉素对Tca8113细胞的毒性作用,阻滞细胞周期进程并诱导细胞凋亡.

Celecoxib enhances the lethal effects of bleomycin in human tongue squamous carcinoma cell line Tca8113

Objective To investigate the role of COX-2 inhibitor celecoxib in enhancing the lethal effects of bleomycin in Tca8113 cell line.Methods Tca8113 ceils were treated with different concentrations of celecoxib and bleomycin for 24, 48, 72 h.Methyl thiazolyl tetrazolium assay was used to calculate cell growth inhibition rate and Jin Zheng Jun's method was used to evaluate the interaction of celecoxib and bleomycin on Tca8113 cells.Flow cytometry was used to evaluate the effects of combined use of celecoxib and bleomycin on cell cycle progress and apoptosis.Results Low dose of celecoxib (10 μmol/L, ＜IC50) combined with bleomycin showed synergism or additive lethal effect on Tca8113 cell line.Celecoxib could notably enhance the inhibitory effect of bleomycin on Tca8113 cells by blocking cell cycle progress and thus resulting in the increasing G0/G1 cells (60.93±0.32) %] distribution and inducing apoptosis (1.87±0.11) %].Conclusions Low doses of celecoxib could significantly enhance the lethal effect of bleomycin on Tca8113 cells by inhibiting cell growth and proliferation through blocking cell cycle progress and inducing apoptosis.The ways of these interactions on inhibiting Tca8113 cell growth were synergistic or/and additive.