雄激素受体、雌激素受体在肝细胞型肝癌中的免疫电镜观察

目的：观察雄激素受体（AR）与雌激素受体（ER）在肝癌和非癌肝组织超微结构上的分布。探讨AR、ER在肝癌致癌过程中的作用。方法：用免疫胶体金染色法（IGS）标记13例肝细胞型肝癌与5例非癌肝组织细胞内雄激素受体（AR）及雌激素受体（ER），并用免疫透射电镜观察。结果：发现胶体金颗粒主要分布于线粒体双层膜和脊膜；粗面内质网；结合及游离核蛋白体；核膜及核周间隙，异染色质及常染色质等部位。统计分析表明，肝癌细胞金颗粒明显多于非癌肝细胞内金颗粒（P＜0．05）。肝癌细胞胞质、胞核AR金颗粒明显多于ER金颗粒（P＜0．05）。而非癌肝细胞胞质、胞核AR、ER金颗粒数量无显著差别（P＞0．05）。结论：提示肝癌是一种雌激素依赖肿瘤，为抗性激素治疗肝癌提供了理论依据。

The observation of androgen receptor and estrogen receptor in hepatocellular carcinomas with immunoelectron - microscopy

Objective :To observe distribution on the ultrastructure of androgen receptor (AR) and estrogen receptor (ER) in hepatocellular carcinomea (Hcc) and non - cancerous liver. To make clear the effect of AR and ER in the course that cause cancer in Hcc. Methods: Using the method of immunogold staining androgen receptor (AR) and estrogen receptor (ER) in 13 cases of hepatocellular carcinomas (Hcc) and 5 cases of noncancerous liver and observing with transmission electron microscope. Results-.Gold granules were mainly distributed over the double - layered membranes of mitochondria,memebranes of rough endoplasmic reticulum and some smooth endoplasmic reticulum,bound and free ribosomes,nuclear membranes and perinuclear cristerna and heterochro- matin and euchromatin, etc. The statistics indicate that the number of gold granules in cancer cells is greater than that in non- cancerous liver cells (P<0.05). In plasma and nucleus of cancer cells, the gold granules of AR is more than those of ER( P < 0.05 ), while no difference exists between plasma and mucleus of non- cancerous liv- er (P >0.05). Conclusion-. It indicates that Hcc is a kind of androgen - depenedent tumor, providing the theo- retical basis for anti - androgen therapy.