| 双抗体夹心酶联免疫法检测不同样品中的蓖麻毒素 |
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| 引用本文： | 郎立伟,王玉霞,王晨宇,赵宇,贾培媛,傅风华.双抗体夹心酶联免疫法检测不同样品中的蓖麻毒素[J].国外医学(药学分册),2009(1):12-16. |
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| 作者姓名： | 郎立伟 王玉霞 王晨宇 赵宇 贾培媛 傅风华 |
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| 作者单位： | 军事医学科学院毒物药物研究所;烟台大学药学院; |
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| 摘 要： | 目的应用酶联免疫吸附分析方法（ELISA）检测待测样品中的蓖麻毒素。方法用蛋白G亲和层析柱纯化蓖麻毒素单克隆抗体（4C13，3D74，5E4和5H6），以3D74及辣根过氧化物酶（HRP）标记的4C13建立的双抗体夹心ELISA对含有蓖麻毒素的多种样品进行检测。结果抗蓖麻毒素的单克隆抗体经亲和层析纯化后具有较高的蛋白纯度，应用HRP标记的4C13与3D74建立双抗体夹心ELISA，对于溶解于磷酸缓冲液中的蓖麻毒素标准品的检测灵敏度可达2．5μg·L^-1；对于土壤、面粉、牛奶、咸菜汁、雪碧、可乐和腐乳汁．中的蓖麻毒素样品检测的灵敏度为2．5-5．0μg·L^-1；与磷酸缓冲液样品相比较，含有相同浓度蓖麻毒素的小鼠和人血清样品ELISA的阳性结果明显减弱．结论双抗体奕心酶联免疫法能够有效用于含有蓖麻毒素样品的检测分析。
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| 关 键 词： | 蓖麻毒素 双抗体夹心 酶联免疫吸附分析 |
Determination of ricin by double antibody sandwich enzyme-linked immunosorbent assay in different samples |
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| LANG Li-wei,WANG Yu-xia,WANG Chen-yu,ZHAO Yu,JIA Pei-yuan,FU Feng-hua.Determination of ricin by double antibody sandwich enzyme-linked immunosorbent assay in different samples[J].Foreign Medical Sciences(Section of Pharmarcy),2009(1):12-16. |
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| Authors: | LANG Li-wei WANG Yu-xia WANG Chen-yu ZHAO Yu JIA Pei-yuan FU Feng-hua |
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| Institution: | LANG Li-wei1,2,WANG Yu-xia1,WANG Chen-yu1,ZHAO Yu1,JIA Pei-yuan1,FU Feng-hua2(1.Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China,2.School of Pharmacy,Yantai University,Yantai 264005,China) |
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| Abstract: | Objective To develop a double antibody sandwich enzyme-linked immunosorbent assay(ELISA) for the determination of ricin.Methods Anti-ricin monoclonal antibodies(4C13,3D74,5E4 and 5H6)were purified on Protein G Sepharose 4 Fast Flow Column.Antibody 4C13 labeled with horseradish peroxidase(HRP) was used to establish the ELISA with 3D74 for ricin detection.Results The antibodies against ricin were highly purified.The double antibody sandwich ELISA was a very sensitive method for the detection of ricin containi... |
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| Keywords: | ricin double antibody sandwich method enzyme-linked immunosorbent assay |
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