尿素酰基裂解酶酶偶联法测定血清总钙

目的 介绍尿素酰基裂解酶酶偶联测定血清总钙的方法。方法 双试剂两点法；缓冲液为三乙醇胺（TEA，pH8.0,200mmol/L）；试剂I：含NaHCO326.0mmol/L,NADPH 0.4mmol/L,α－酮戊二酸8.0mmol/L，尿素酰基裂解酶115U／L，GLDH43．0kU/L；试剂Ⅱ：含NaCO3 26.0mmol/L,ATP6.2mmol/L。结果 方法线性范围为0．5－5．0mmol/L，批内和批间平均变异系数分别为2．13％和2．69％。平均回收率为102．7％ 。本法（Y）与邻甲酚酞络合酮法（OCPC法，X1）比较：r＝0．981，Y1＝1．02X1－0．021，与偶氮胂Ⅲ法（X2）比较：r＝0．978，Y＝0．99X2＋0．03。血清胆红素高达300μmol/L，血红蛋白4g/L，镁2.5mmol/L，NH4^ 2.0mmol/L,Trig 8.0mmol/L对本法无明显干扰。结论 本法具有简便、准确、快速，适用于自动分析等特点。

A enzymatic method of the determination of calcium in serum

Objective A enzymatic method for measurement of calcium in serum by using urea amidolyase was introduction. Methods Double reagent two-point method.TEA buffer(pH 8.0,200 mmol/L)was used as the buffer solution.The first reagent consisted of sodium bicarbonate 26.0 mmol/L,NADPH 0.4 mmol/L,2-oxoglutaric acid 8.0 mmol/L,urea amidolyase 115 U/L and GLDH 43.0 kU/L.The second reagent contained sodium bicarbonate 26.0 mmol/L and ATP 6.2 mmol/L in the buffer solution.Results The Linearity range was 0.5～5.0 mmol/L.The average rate of recovery was 102.7%.The average within-run and between-run CV were 2.13% and 2.69%.Camparison with OCPC(X1)，showed that Y1=1.02X1-0.021,r=0.981,and with Arsenazo Ⅲ(X2),Y2=0.99X2+0.03,r=0.978.No interference from as much as 300 μmol/L of bilirubin,4 g/L of hemoglobin,2.5 mmol/L of magnesium,2.0 mmol/L of ammonium ion and 8.0 mmol/L of triglyceride.Conclusion This method was simple,accurate and ripid,suitable for automatic analysis of calcium in serum.