胰岛素促进体外培养的小鼠卵巢颗粒细胞增殖

目的：研究胰岛素对体外培养的小鼠卵巢颗粒细胞增殖的影响，探讨胰岛素的促增殖作用是否与浓度相关。方法：未成熟小鼠卵巢颗粒细胞原代培养，四甲基偶氮唑蓝法（MTT）测定颗粒细胞增殖指数，观察不同浓度胰岛素对颗粒细胞增殖的影响。结果：与对照组相比，5ng·ml^-1。胰岛素对颗粒细胞没有促增殖作用（P〉0.05），10ng·ml^-1有促增殖作用（P〈0.05），100ng·ml^-1、1000ng·ml^-1的促增殖作用更强（P〈0.01），100ng·ml^-1、1000ng·ml^-1作用无明显差异（P〉0.05）。结论：生理浓度的胰岛素能促进颗粒细胞增殖，高浓度胰岛素促增殖作用更强，本实验为进一步研究高胰岛素血症在多囊卵巢综合症（PCOS）的卵泡发育停滞机制奠定基础。

Insulin promotes the proliferation of mice granule cells cultured in vitro

Objective： To study the effect of insulin on the proliferation of mice granule cells, and to discuss the relationship between the effect and the concentration of insulin Methods： The primary model of cultured mice granule cells in vitro was established and the cells were treated with different concentration of insulin. The degree of proliferation of granule cells was determined by MTT. Results： Compared with the control group, insulin did not promote the proliferation of granule cells at a concentration of 5 ng·ml^-1 （P〉0. 05）, it had the effect of promotion at the concentration of 10 ng·ml^-1（P〈0.05） ,with increasing concentration of 100,1000 ng·ml^-1, the effect of insulin was increased （P〈0.01）. Conclusion： Insulin can promote the proliferation of granule cells at physiological concentration. When the concentration of insulin increased, the effect was increased. The study settled the foundation for future research about hyperinsulinemia in follicular failure to development in PCOS.