| 同位素示踪沉淀法测定神经生长因子血药浓度 |
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| 作者姓名： | Tang G Jiang G Zhang Y Chen L Wang S |
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| 作者单位： | 1. 中国医学科学院中国协和医科大学协和医院,北京 100730
2. 中国医学科学院中国协和医科大学基础医学研究所 |
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| 摘 要： | 目的 建立测定小鼠血浆神经生长因子（ＮＧＦ）浓度的同位素示踪沉淀法。方法 采用放射性同位素（＾１２５Ｉ）标记示踪法结合三氯醋酸（ＴＣＡ）沉淀法测定ＮＧＦ的浓度。结果 本方法的最低检测浓度为２．０ｎｇ／ｍｌ,日内、日间变异系数（ＣＶ）均小于１０％,血浆中ＮＧＦ在１２．５－４００ｎｇ／ｍｌ浓度范围内均呈良好的相关性,相关系数ｒ＝０．９９９０,血浆中ＮＧＦ的平均回收率为９５．２１％,。结论 同位素标记示
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| 关 键 词： | 神经生长因子 同位素 示踪标记法 TCA沉淀法 |
| 修稿时间： | 1997年3月20日 |
Isotope tracer combined with trichloroacetic acid precipitation method for determination of nerve growth factor concentration in mouse plasma |
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| Tang G,Jiang G,Zhang Y,Chen L,Wang S.Isotope tracer combined with trichloroacetic acid precipitation method for determination of nerve growth factor concentration in mouse plasma[J].Acta Academiae Medicinae Sinicae,1998,20(3):203-206. |
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| Authors: | Tang G Jiang G Zhang Y Chen L Wang S |
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| Institution: | PUMC Hospital, CAMS and PUMC, Beijing 100730. |
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| Abstract: | OBJECTIVE: To establish the radioisotope (125I) tracer method combined with trichloroacetic acid (TCA) precipitation for the determination of nerve growth factor (NGF) in mouse plasma. METHODS: Radioisotope tracer labelling method combined with TCA precipitation was used to determine NGF in mouse plasma. RESULTS: The detection limit of the method for NGF in plasma was 2.0 ng/ml. The intra-assay cv (CV) and the inter-assay CV were all less than 10%. The assay linearity was determined over the range of 12.5-400 ng/ml in plasma and the correlation coefficient was 0.9990. The average recovery of the method for NGF was 95.21%. CONCLUSIONS: The radioisotope tracer method combined with TCA precipitation is stable, sensitive and reliable. It is suitable for the determination of NGF concentration in mouse plasma. |
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