p63蛋白在非小细胞肺癌中的表达及意义

目的 :研究p6 3蛋白的表达与非小细胞肺癌临床病理学特征的关系。方法 :随机收集非小细胞肺癌标本 4 0例 (其中鳞癌 18例、腺癌 2 2例 ;15例伴淋巴结转移 ,2 0例无淋巴结转移 )及 7例肺癌患者正常肺组织标本 ,采用免疫组织化学S P法检测p6 3蛋白的表达并结合肺癌的临床病理学特征进行分析。结果 :p6 3蛋白的表达在非小细胞肺癌细胞和正常支气管粘膜上皮细胞中定位于细胞核 ,呈棕黄色。在肺鳞癌、肺腺癌、正常支气管粘膜上皮细胞中 ,p6 3阳性表达率分别为 77.8% (14 18)、2 7.3% (6 2 2 )、2 8.6 % (2 7) ,肺鳞癌分别与肺腺癌和正常支气管粘膜上皮相比较差异均有高度显著性 (P <0 .0 1) ;而肺腺癌和正常支气管粘膜上皮比较差异无显著性 (P >0 .0 5 ) ;p6 3蛋白表达与非小细胞肺癌分化程度、临床分期、淋巴结转移无统计学意义。结论 :p6 3蛋白在肺鳞癌中表达明显上调。

Expression of p63 protein in Non-small Cell Lung Cancer and Its Clinicopathological Significance

Objective: To investigate p63 protein expression and the relationship with clinicopathlological features in non small cell lung cancer (NSCLC). Methods: 40 cases of NSCLC and 7 cases of normal lung tissues were selected randomly. These samples were detected by immunohistochemical technique, and the relationship with clinicopathological features were analyzed. Results: p63 protein expression was localized in nucleus of NSCLC cells, showing brownish yellow. The positive rates of p63 expression were: lung squamous cell cancer 77.8% (14/18), lung adenocarcinomas 27.3%(6/22), normal epithelial cell of bronchiole 28.6%(2/7), respectively. A higher positive rates of p63 expression was found in lung squamous cell cancer compared with lung adenocarcinomas and normal epithelial cells of bronchiole (P<0.01). No significant difference was observed between lung adenocarcinomas and normal epithelial cells of bronchiole (P>0.05). The expression of p63 protein was not related to tumor differentiation degree,lymph node metastasis and tumor stage. Conclusion: Overexpression of p63 protein was found in lung squamous cell cancer.