重症肌无力大鼠膈肌运动终板的形态学研究

目的: 探讨重症肌无力大鼠膈肌运动终板的光镜及电镜结构变化.方法: 被动转移乙酰胆碱受体单抗mAb35建立SD大鼠获得性自身免疫性重症肌无力模型,用乙酰胆碱酯酶法显示膈肌运动终板,并行超微结构观察.结果: 实验组大鼠注射mAb35后出现明显肌无力症状,其膈肌运动终板数量基本不变,但出现与重症肌无力患者相似的超微结构变化,对照组则无明显变化.结论: 膈肌出现运动终板超微结构的改变,是重症肌无力被动转移模型大鼠呼吸衰竭的可能原因.

Research on morphological changes of end plates in diaphragm of experimental autoimmune myasthenia gravis rats

AIM: To study the pathological characteristics of the end plate on diaphragm in experimental autoimmune myasthenia gravis animal model by light microscopy and electron microscopy. METHODS: Passively transferred myasthenia gravis animal model was prepared by injection of acetylcholine receptor monoclonal antibody mAb35, and the ultrastructure of the end plates was studied by acetylcholinesterase histochemical technique. RESULTS: Myasthenia symptoms were induced in the experimental group. The end plates of diaphragm showed ultrastructural changes similar to those of myasthenia gravis patients but no change was found in the number of the end plates. No changes were observed in the control group. CONCLUSION: It is suggested that the ultrastructural changes of the end plates on diaphragm may result in the dyspnea in experimental autoimmune myasthenia gravis.