Cytotoxic and anti-inflammatory effects of onion peel extract on lipopolysaccharide stimulated human colon carcinoma cells |
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| Affiliation: | 1. Faculty of Pharmacy, Department of Pharmacology and Therapeutics, Kuwait University, PO Box 24923, Safat, 13110, Kuwait;2. Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Kuwait University, PO Box 24923, Safat, 13110, Kuwait;1. School of Environment, University of the Aegean, Mitr. Ioakim Street, Myrina 81400, Lemnos, Greece;2. Food Quality & Chemistry of Natural Products, Mediterranean Agronomic Institute of Chania (M. A. I. Ch.), International Centre for Advanced Mediterranean Agronomic Studies (CIHEAM), P.O. Box 85, Chania 73100, Greece;1. Division of Livestock Products Technology, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India;2. Department of Food Technology, Faculty of Science and Humanities, SRM University, Sonipat, Haryana 131 029, India;3. Department of Botany and Microbiology, Gurukula Kangri (Deemed to be University), Haridwar, Uttarakhand 249 404, India;4. Department of Food Engineering and Technology, Tezpur University, Tezpur, Assam 784 028, India;5. Division of Food Technology, Department of Nutrition and Dietetics, Manav Rachna International Institute of Research and Studies, Faridabad, Haryana 121 004, India;6. Department of Botany, Rotary Institute of Management and Technology, Chandausi, Uttar Pradesh 244 412, India;7. Livestock Production and Management Section, ICAR-Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122, India |
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| Abstract: | The present study investigated the cytotoxic activity of ethanol extract of onion peel (OPE) in HT-29 human colon carcinoma cells. High-performance liquid chromatography (HPLC) analysis was performed to determine the amounts of phenolic acids and flavonoids in OPE. In addition, the influence of OPE on antioxidant- and inflammation-associated gene expression was also determined in a model of lipopolysaccharide (LPS)-stimulated HT-29 cells. HPLC analysis showed that OPE contained well-known antioxidant compounds, including p-coumaric acid, vanillic acid, epicatechin, and morin. After incubation with OPE, HT-29 cells showed either a loss of normal nuclear architecture or detachability from each other. The cytotoxic effects of OPE on HT-29 cells were confirmed by MTT and LDH release assays. LPS-induced oxidative conditions effectively downregulated TNF-α mRNA expression in OPE pretreated HT-29 cells compared with cells only stimulated with LPS. In addition, the expression of heme oxygenase-1 (HO-1) and glutathione S-transferase (GSTs) detoxification genes (i.e., GSTM1, GSTT1, and GSTP1) was upregulated after treatment with LPS at sublethal concentrations. However, the LPS-induced mRNA expression of HO-1 and GSTs was significantly attenuated by treatment with OPE. Therefore, onion peel extract is a promising component of future nutraceuticals and value-added products. |
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| Keywords: | Onion peel Cell proliferation Lipopolysaccharide Human colon carcinoma cells |
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