Recombinant Expression of Trametes versicolor Aflatoxin B1-Degrading Enzyme (TV-AFB1D) in Engineering Pichia pastoris GS115 and Application in AFB1 Degradation in AFB1-Contaminated Peanuts |
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| Authors: | Peizhou Yang Wei Xiao Shuhua Lu Suwei Jiang Zhi Zheng Danfeng Zhang Min Zhang Shaotong Jiang Shuying Jiang |
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| Institution: | 1.Anhui Key Laboratory of Intensive Processing of Agricultural Products, College of Food and Biological Engineering, Hefei University of Technology, 420 Feicui Road, Shushan District, Hefei 230601, China; (W.X.); (S.L.); (Z.Z.); (D.Z.); (M.Z.); (S.J.); (S.J.);2.School of Biological, Food and Environment Engineering, Hefei University, 158 Jinxiu Avenue, Hefei 230601, China; |
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| Abstract: | Aflatoxins seriously threaten the health of humans and animals due to their potential carcinogenic properties. Enzymatic degradation approach is an effective and environmentally friendly alternative that involves changing the structure of aflatoxins. In this study, Trametes versicolor aflatoxin B1-degrading enzyme gene (TV-AFB1D) was integrated into the genome of Pichia pastoris GS115 by homologous recombination approach. The recombinant TV-AFB1D was expressed in engineering P. pastoris with a size of approximately 77 kDa under the induction of methanol. The maximum activity of TV-AFB1D reached 17.5 U/mL after the induction of 0.8% ethanol (v/v) for 84 h at 28 °C. The AFB1 proportion of 75.9% was degraded using AFB1 standard sample after catalysis for 12 h. In addition, the AFB1 proportion was 48.5% using AFB1-contaminated peanuts after the catalysis for 18 h at 34 °C. The recombinant TV-AFB1D would have good practical application value in AFB1 degradation in food crops. This study provides an alternative degrading enzyme for the degradation of AFB1 in aflatoxin-contaminated grain and feed via enzymatic degradation approach. |
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| Keywords: | aflatoxin B1 Pichia pastoris GS115 peanuts Trametes versicolor degradation enzyme |
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