Urinary analysis reveals high deoxynivalenol exposure in pregnant women from Croatia |
| |
| Institution: | 1. Subdepartment of Biochemistry and Toxicology, Department of Applied Chemistry and Ecology, Faculty of Food Technology, Josip Juraj Strossmayer University, Osijek, Croatia;2. Center for Analytical Chemistry, Department for Agrobiotechnology (IFA-Tulln), University of Natural Resources and Life Sciences, Vienna (BOKU), Austria;3. Section for Chemistry and Toxicology, Norwegian Veterinary Institute, Oslo, Norway;4. Laboratory of Pharmacology and Toxicology, Department of Biochemistry, Faculty of Science, University of Yaounde I, Cameroon;5. Department of Food Technology, Faculty of Science, University of Johannesburg, 2028, Doornfontein Campus, P.O. Box 17011, Gauteng, South Africa;6. Subdepartment of Nutrition, Department of Food and Nutrition Research, Faculty of Food Technology, Josip Juraj Strossmayer University, Osijek, Croatia;1. College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, Jiangsu, China;2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, Jiangsu, China;3. Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, 225009, Jiangsu, China;1. Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Franje Kuha?a 18, HR 31000 Osijek, Croatia;2. University of Zagreb, Faculty of Agriculture, Sveto?imunska cesta 25, HR 10000 Zagreb, Croatia;1. Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, F. Kuha?a 20, 31000 Osijek, Croatia;2. Scientific Veterinary Institute Novi Sad, Rumena?ki put 20, 21000 Novi Sad, Serbia;3. University of Novi Sad, Faculty of Agriculture, Trg Dositeja Obradovi?a 8, 21000 Novi Sad, Serbia;4. The Faculty of Agriculture and Food Technology (APTF) of the University of Mostar, Biskupa ?ule bb, 88000 Mostar, Bosnia and Herzegovina;1. Vaccine Immunology Laboratory, OSPC Division, CSIR-Indian Institute of Chemical Technology, Hyderabad 500007, India;2. Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, Uttar Pradesh 201 002, India;3. Department of Chemistry, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi, India |
| |
| Abstract: | In this pilot survey the levels of various mycotoxin biomarkers were determined in third trimester pregnant women from eastern Croatia. First void urine samples were collected and analysed using a “dilute and shoot” LC–ESI–MS/MS multi biomarker method. Deoxynivalenol (DON) and its metabolites: deoxynivalenol-15-glucuronide and deoxynivalenol-3-glucuronide were detected in 97.5% of the studied samples, partly at exceptionally high levels, while ochratoxin A was found in 10% of the samples. DON exposure was primarily reflected by the presence of deoxynivalenol-15-glucuronide with a mean concentration of 120 μg L?1, while free DON was detected with a mean concentration of 18.3 μg L?1. Several highly contaminated urine samples contained a third DON conjugate, tentatively identified as deoxynivalenol-7-glucuronide by MS/MS scans. The levels of urinary DON and its metabolites measured in this study are the highest ever reported, and 48% of subjects were estimated to exceed the provisional maximum tolerable daily intake (1 μg kg?1 b.w.). |
| |
| Keywords: | Mycotoxins Deoxynivalenol glucuronide Biomarker Pregnant women Human urine Croatia |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
