Free radical scavenging and anti-oxidative activities of an ethanol-soluble pigment extract prepared from fermented Zijuan Pu-erh tea |
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| Institution: | 1. College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China;2. Xiamen Amoytop Biotech Co., Ltd., Xiamen 361022, China;1. Clinical and Translational Epidemiology Unit, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts;3. Division of Gastroenterology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts;4. Department of Medicine, Clinical Epidemiology Unit, Karolinska Institutet, Stockholm, Sweden;6. Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Stockholm, Sweden;5. Department of Pediatrics, Örebro University Hospital, Örebro University, Örebro, Sweden;7. Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health, Massachusetts;12. Channing Division of Network Medicine, Department of Medicine, Brigham and Women''s Hospital and Harvard Medical School, Boston, Massachusetts;1. Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091, China;2. College of Forestry, Northwest A & F University, 3 Taicheng Road, Yangling 712100, China;1. National Institute of Materials Physics, 077125, Magurele, Romania;2. Faculty of Physics, University of Bucharest, 077125, Magurele, Romania;3. Politehnica University of Bucharest, Faculty of Applied Chemistry and Material Science, Department of Oxide Materials and Nanomaterials, 011061, Bucharest, Romania;1. Cancer and Inflammation Program, Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, National Cancer Institute, Frederick, MD 21702, USA;2. Sackler Inst. of Molecular Medicine, Department of Human Genetics and Molecular Medicine, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel;1. Research Center for Combustion and Environment Technology, Shanghai Jiao Tong University, Shanghai 200240, PR China;2. Key Laboratory for Power Machinery and Engineering of Ministry of Education, Shanghai Jiao Tong University, Shanghai 200240, PR China;1. Department of Food Science and Human Nutrition, College of Applied and Health Sciences, A''Sharqiah University, Ibra, Oman;2. Department of Chemistry, College of Science, Sultan Qaboos University, PO Box 36, Al-Khod 123, Muscat, Oman;3. Biomolecular NMR, Ruhr University of Bochum, 44780 Bochum, Germany;4. Inorganic Chemistry 1, Ruhr-University Bochum, Universitätsstrasse 150, 44801 Bochum, Germany |
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| Abstract: | An ethanol-soluble pigment extract was separated from fermented Zijuan Pu-erh tea. The compositions of the ethanol soluble pigment extract were analyzed by high-performance liquid chromatography–tandem mass spectroscopy (HPLC–MS/MS). The extract was prepared into a series of ethanol solutions and analyzed for free radical-scavenging activities (against two free radicals: 1,1-diphenyl-2-picrylhydrazyl (DPPH) and (2,2,6,6-tetramethylpiperidin-1-yl)oxyl (TEMPO)) and in vitro anti-oxidative properties. Electron spin resonance spectroscopy showed that the peaks of DPPH and TEMPO decreased with increasing extract concentration, suggesting that the extract had excellent free radical-scavenging activities. In vitro cell culture suggested that, at 50–200 mg/L, the extract had no measurable effect on the viability of vascular endothelial cells (ECV340) but produced significant protective effects for cells that underwent oxidative injuries due to hydrogen peroxide (H2O2) treatment. Compared with the H2O2 treatment alone cells group, 200 mg/L of the extract increased the activity of superoxide dismutase (SOD) in cells by 397.3%, and decreased the concentration of malondialdehyde (MDA) and the activity of lactate acid dehydrogenase (LDH) by 47.8% and 69.6%, respectively. These results suggest that the extract has excellent free radical scavenging and anti-oxidative properties. |
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| Keywords: | Zijuan Pu-erh tea Ethanol-soluble pigment Free radical Anti-oxidation |
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