Anti-inflammatory activity of hexane extracts from bones and internal organs of Anguilla japonica suppresses cyclooxygenase-2-dependent prostaglandin D2 generation in mast cells and anaphylaxis in mice |
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| Affiliation: | 1. College of Pharmacy, Yeungnam University, Gyeongsan 712-749, Republic of Korea;2. College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea;3. Department of Biological Science, Sungkyunkwan University, Suwon 440-746, Republic of Korea;4. Department of Food Science and Biotechnology, Kyung Sung University, Busan 608-736, Republic of Korea;5. Department of Pathology, Catholic University of Daegu School of Medicine, Daegu 705-718, Republic of Korea;6. Lipid Metabolism Project, The Tokyo Metropolitan Institute of Medical Science, 2-1-6 Kamikitazawa, Setagaya-ku, Tokyo 156-8506, Japan;1. Department of Medical, Surgical and Experimental Sciences, Section of Pharmacology, University of Sassari, V. le S. Pietro 43/B, 07100, Sassari, Italy;2. Department of Chemistry and Pharmacy, University of Sassari, via Muroni 23, 07100 Sassari, Italy;3. Department of Life and Environmental Sciences and Centre of Excellence on Neurobiology of Addiction, University of Cagliari, University Campus, I-09042, Monserrato, Cagliari, Italy;1. Department of Food Science and Biotechnology, Kunsan National University, Kunsan 54150, South Korea;2. Biotechnology Research Division, Aquaculture Industry Department, National Institute of Fisheries Science, Busan 46083, South Korea;3. Department of Biotechnology, Pukyong National University, Busan 48513, South Korea;4. Department of Marine Biotechnology, Kunsan National University, Kunsan 54150, South Korea;5. Department of Marine Aquaculture and Aquatic Sciences, Kunsan National University, Kunsan 54150, South Korea;6. Department of Chemistry, Kunsan National University, Kunsan 54150, South Korea;1. Department of Biotechnology, Pukyong National University, Busan 608-737, Republic of Korea;2. Biotechnology Research Division, National Fisheries Research & Development Institute, Busan 619-705, Republic of Korea;3. Department of Oral Biochemistry, Dental Science Research Institute, Medical Research Center for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju, Republic of Korea;1. Behavioral Neuroscience Program, Department of Psychology, Binghamton University, Binghamton, NY, USA;2. Department of Psychology, Ithaca College, Ithaca, NY, USA;1. Division of Allergic Diseases, Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota;2. Division of Allergy and Immunology, Department of Pediatrics, Mayo Clinic, Rochester, Minnesota;3. Department of Pediatrics, University of Missouri–Kansas City, Faculty, Division of Allergy, Asthma, and Immunology, Children''s Mercy Hospital, Kansas City, Missouri |
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| Abstract: | The purpose of this study is to investigate the effects of n-hexane extracts from bones and internal organs of Japanese eel, Anguilla japonica (HEE), on cyclooxygenase-2 (COX-2)-dependent prostaglandin D2 (PGD2) generation in stem cell factor (SCF), IL-10, plus LPS-induced mouse bone marrow-derived mast cells (BMMCs) and on passive cutaneous anaphylaxis (PCA) in mice. HEE suppressed SCF/IL-10/LPS-induced PGD2 generation, and concomitantly reduced COX-2 protein expression dose-dependently. To understand the mechanistic basis for the inhibition of PGD2 generation by HEE, we examined the effects of HEE on upstream signaling pathways essential for COX-2 induction. HEE was found to inhibit the translocation of nuclear factor-κB (NF-κB) p65 subunit to the nucleus and its DNA-binding ability through the inhibition of TAK1, IKK and IκB phosphorylation.Furthermore, HEE also attenuated mitogen-activated protein kinase (MAPK)-mediated regulation of DNA binding of activator protein-1 (AP-1). Moreover, oral administration of HEE inhibited anti-dinitrophenyl (DNP) IgE-induced PCA in a dose dependent manner. Taken together, the present study provides new insights into the anti-inflammatory activity of HEE, which could be a promising candidate to be used for an inflammatory therapy. |
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| Keywords: | Japanese eel Mast cells Mitogen-activated protein kinase Nuclear factor-κB Passive cutaneous anaphylaxis |
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