Screening of Lactobacillus strains for their ability to bind Benzo(a)pyrene and the mechanism of the process |
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| Affiliation: | 1. College of Biological Science & Biotechnology, Beijing Forestry University, 100083 Beijing, China;2. Engineering & Technology Research Center of Food Preservation, Processing & Safety Control of Liaoning Province, Bohai University, 121000 Jinzhou, Liaoning, China;3. Institute of Fermentation Technology & Microbiology, Technical University of Lodz, 90924 Lodz, Poland;1. State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, PR China;2. Beijing Innovation Centre of Food Nutrition and Human Health, Beijing Technology & Business University, Beijing 100048, PR China;3. UK-China Joint Centre on Probiotic Bacteria, United Kingdom;1. Universidade Estadual de Londrina, Laboratory of Animal Pathology, Campus Universitário, Rodovia Celso Garcia Cid, Km 380, Londrina, Paraná 86051-990, Brazil;2. Université de Toulouse, Toxalim (Research Center in Food Toxicology), INRA, ENVT, INP-PURPAN, UPS, Toulouse, France;3. Université Saint-Joseph, Centre d’Analyses et de Recherches (Faculté des Sciences), Campus des Sciences et Technologies, Mar Roukos, Mkallès, P.O Box 11- 514 Riad El Solh, Beyrouth 1107 2050, Lebanon;1. Departamento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil;2. Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil;1. Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán (UNT), Ayacucho 471, 4000 Tucumán, Argentina;2. Centro Científico Tecnológico Tucumán–Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Ayacucho 471, 4000 Tucumán, Argentina |
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| Abstract: | In order to investigate the binding ability of Lactobacillus strains to Benzo(a)pyrene (BaP), 15 strains were analysed. L. plantarum CICC 22135 and L. pentosus CICC 23163 exhibited high efficiency in removing BaP from aqueous medium; the binding rates were 66.76% and 64.31%, respectively. This process was affected by temperature, incubation time and pH, and cell viability was not necessary for the binding ability. Additionally, both strains, especially strain CICC 23163 showed high specificity in binding BaP. The cell-BaP complexes were stable in aqueous medium. The mechanism of binding was investigated by examining the binding ability of different components of the microorganism cells. The results revealed that peptidoglycans played an important role in binding BaP and its structural integrity was required. Consequently, we proposed that the mechanism of this process was a physisorption and peptidoglycan was the main binding site. These two strains may be used for dietary detoxification in human diet and animal feed. |
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| Keywords: | Benzo(a)pyrene binding mechanism |
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