Comparison of anticancer activity between lactoferrin nanoliposome and lactoferrin in Caco-2 cells in vitro |
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| Affiliation: | 1. Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom;2. College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom;1. Department of Applied Prosthodontics, Institute of Biomedical Sciences, Nagasaki University, 1-7-1, Sakamoto, Nagasaki 852-8588, Japan;2. Department of Dental and Biomaterials Science, Institute of Biomedical Sciences, Nagasaki University, 1-7-1, Sakamoto, Nagasaki 852-8588, Japan;2. Centre of Biological Engineering, University of Minho, Braga, Portugal;3. Instituto de Biologia Experimental e Tecnológica, Avenida da República, Quinta-do-Marquês, Estação Agronómica Nacional, Oeiras, Portugal;1. Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada (Spain), Avda, Ilustración, 60, 18016, Spain;2. Instituto Investigación Biosanitaria, ibs.Granada, C/Dr. Azpitarte 4 - 4ª planta, 18012 Granada, Spain;3. Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences (Melilla), University of Granada (Spain). Camino Cdad, De Málaga 22, 52005, Melilla, Spain;4. Institute of Neuroscience Federico Olóriz, University of Granada, (Spain), Avda. del Conocimiento S/N, 18016, Armilla (Granada), Spain |
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| Abstract: | The anticancer activities of Lactoferrin (Lf) and Lf nanoliposomes in Caco-2 cells were observed in this study, and mitochondrial function (MTT assay), count kit-8(CCK-8), detection of intracellular reactive oxygen species (ROS) and apoptosis induction (AO/EB staining) assays were used to evaluate the anticancer activity. MTT results demonstrated that Lf nanoliposomes and Lf reduced the mitochondrial activity of cells in a manner of dose and time effect, and the viabilities of Caco-2 cell were significantly decreased in vitro following exposure to Lf nanoliposomes at the concentrations of 5 and 10 mg/mL. LDH leakage and ROS significantly increased in cells exposed to Lf nanoliposomes (⩾5 mg/mL), while Lf induced ROS only at higher doses (10 mg/mL). CCK-8 evaluation of cell proliferation and AO/EB double staining supported the anti-proliferative effects of Lf liposomes. Our findings demonstrated that the presence of Lf nanoliposome is more significant than Lf in inhibiting human tumor cells proliferation. Therefore, it can be concluded that Lf nanoliposomes are a potential therapeutic modality in the management of tumors. |
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| Keywords: | Lactoferrin nanoliposomes Caco-2 Cell viability MTT |
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