Protective effect of extract of Crataegus pinnatifida pollen on DNA damage response to oxidative stress |
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| Affiliation: | 1. Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, PR China;2. Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, PR China;1. Grupo Multidisciplinar de Saúde (GMS), Universidade de Cruz Alta (UNICRUZ), 98020-290 Cruz Alta, RS, Brazil;2. Grupo Interdisciplinar de Saúde (GIS), Centro de Ensino e Pesquisa (CEP), Instituto de Cardiologia de Cruz Alta (ICCA), 98005-096 Cruz Alta, RS, Brazil;3. Laboratório de Bioquímica Clínica, Departamento de Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, 97105-900 Santa Maria, RS, Brazil;4. Centro de Ciências Biológicas e da Saúde, Pontifícia Universidade Católica do Paraná (PUC-PR), 80215-901 Curitiba, PR, Brazil |
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| Abstract: | The protective effect of extract of Crataegus pinnatifida (Rosaceae) pollen (ECPP) on the DNA damage response to oxidative stress was investigated and assessed with an alkaline single-cell gel electrophoresis (SCGE) assay and pBR322 plasmid DNA breaks in site-specific and non-site-specific systems. Total phenolic content, total flavonoid content, individual phenolic compounds, antioxidant activities (1,1-diphenyl-2-picrylhydrazyl (DPPH), radical scavenging activity, FRAP, and chelating activity) were also determined. The results showed that ECPP possessed a strong ability to protect DNA from being damaged by hydroxyl radicals in both the site-specific system and the non-site-specific system. It also exhibited a cytoprotection effect in mouse lymphocytes against H2O2-induced DNA damage. These protective effects may be related to its high total phenolic content (17.65 ± 0.97 mg GAE/g), total flavonoid content (8.04 ± 0.97 mg rutin/g), strong free radical scavenging activity and considerable ferrous ion chelating ability (14.48 ± 0.21 mg Na2EDTA/g). |
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| Keywords: | DNA damage Oxidative stress Phenolic compounds |
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