| HPLC法测定绞股蓝总苷胶囊中绞股蓝皂苷B的含量 |
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| 引用本文： | 刘春祥,覃玉梅,张群,官启嘉,谢二磊,周国平. HPLC法测定绞股蓝总苷胶囊中绞股蓝皂苷B的含量[J]. 药物分析杂志, 2012, 0(9): 1611-1613,1616 |
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| 作者姓名： | 刘春祥 覃玉梅 张群 官启嘉 谢二磊 周国平 |
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| 作者单位： | 江西省药品认证中心;江西省食品药品检验所 |
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| 基金项目： | 江西省科技支撑计划项目(2010BSA17700) |
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| 摘 要： | 目的:建立反相高效液相色谱法测定绞股蓝总苷胶囊中绞股蓝皂苷B的含量。方法:采用Agilent Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.5%磷酸(38∶62)为流动相,流速1.0 mL.min-1,检测波长210 nm,柱温30℃。结果:绞股蓝皂苷B进样量在0.04～2.0μg范围内,与峰面积呈良好线性关系;平均回收率(n=6)为102.1%,RSD为1.2%。结论:该方法简便、准确,重复性好,可作为绞股蓝总苷胶囊的质量控制方法。
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| 关 键 词： | 绞股蓝总苷胶囊 绞股蓝(七叶胆) 达玛烷型皂苷类成分 绞股蓝皂苷B 高效液相色谱法 不同批次含量差异 中药制剂质量控制 |
HPLC determination of gypenoside B in Jiaogulan Zonggan capsules |
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| LIU Chun-xiang,QIN Yu-mei,ZHANG Qun,GUAN Qi-jia,XIE Er-lei,ZHOU Guo-ping. HPLC determination of gypenoside B in Jiaogulan Zonggan capsules[J]. Chinese Journal of Pharmaceutical Analysis, 2012, 0(9): 1611-1613,1616 |
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| Authors: | LIU Chun-xiang QIN Yu-mei ZHANG Qun GUAN Qi-jia XIE Er-lei ZHOU Guo-ping |
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| Affiliation: | 1.Jiangxi Provincial Certification Committee for Drugs,Nanchang 330046,China; 2.Jiangxi Provincial Institute for Food and Drug Control,Nanchang 330029,China) |
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| Abstract: | Objective:To develop a RP-HPLC method for determination of gypenoside B in Jiaogulan Zonggan capsules.Methods:The separation was performed at 30 ℃ on an Agilent Eclipse XDB-C18(250 mm×4.6 mm,5 μm)column with a mobile phase of acetonitrile-0.5% phosphoric acid(38∶ 62).The flow rate was 1.0 mL·min-1,and the detection wavelength was 210 nm.Results:The linear range of gypenoside B was 0.04-2.0 μg;The average recovery(n=6)of gypenoside B was 102.1%(RSD=1.2%).Conclusions:The method is simple and accurate with good reproducibility,which can be used for the quality control of Jiaogulan Zonggan capsules. |
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| Keywords: | Jiaogulan Zonggan capsules Gnostemma pentaphyllum(Thunb.)Makino dammarane type saponins gypenoside B HPLC different batches content difference Chinese materia medica preparation quality control |
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