精子DNA损伤、核蛋白组型转换与顶体酶活性及精液参数的相关性分析

目的:探讨精子DNA损伤、精子核蛋白组型转换与顶体酶活性及精液参数的相关性。方法:收集535例精液标本,采用精子染色质扩散(sperm chromatin dispersion,SCD)检测精子DNA损伤,并与精子核蛋白组型转换、顶体酶活性和WHO手册(第4版)精液参数进行相关性分析。结果:精子DNA损伤与精子核蛋白组型转换、顶体酶活性、精子浓度及前向运动精子这些指标之间比较均具有显著性差异(P<0.01);精子DNA损伤与年龄、核蛋白组型转换、精子浓度和D级精子比例之间呈显著正相关(P<0.01或P<0.05),而与顶体酶活性呈显著负相关(P<0.001),多元逐步回归分析显示年龄、精子浓度、D级精子比例、核蛋白组型转换及顶体酶活性是5个独立的相关变量。精子核蛋白组型转换、顶体酶活性、精子密度和前向运动精子这4个指标的异常率在精子DNA异常组(DFI≥30%)中均显著的高于正常组(DFI<30%)。结论:精子DNA损伤与精子核蛋白组型转换、顶体酶活性及WHO(第4版)精液各参数之间存在密切的联系,可能是评价精子质量的另一项重要的指标。

Sperm DNA damage and sperm-nucleoprotein transition correlate to acrosin activity and seminal parameters

Objective: To investigate the correlation of sperm DNA damage and sperm-nucleoprotein transition with acrosin activity and seminal parameters.Methods: We collected 535 semen samples,assessed sperm DNA damage by sperm chromatin dispersion test,and analyzed the correlation of sperm DNA damage and sperm-nucleoprotein transition with acrosin activity and seminal parameters according to the WHO criteria.Results: Statistically significant differences were observed in sperm DNA damage among sperm-nucleoprotein transition,acrosin activity,sperm concentration and the percentage of grade a + b sperm(P < 0.01).Sperm DNA damage was positively correlated with age,sperm-nucleoprotein transition,sperm concentration and the percentage of grade d sperm(P < 0.01 or P < 0.05),but negatively correlated with acrosin activity(P < 0.001).Stepwise linear regression analysis demonstrated that age,sperm concentration,the percentage of grade d sperm,sperm-nucleoprotein transition and acrosin activity were independent variables related to the DNA fragmentation index(DFI).The abnormality rates of sperm-nucleoprotein transition,acrosin activity,sperm concentration and graded a + b sperm were significantly higher in the sperm DNA damage group(DFI ≥ 30%) than in the normal control(DFI < 30%)(P < 0.01).Conclusion: Sperm DNA damage is closely related with sperm-nucleoprotein transition,acrosin activity and seminal parameters,which may become another important independent parameter for the evaluation of sperm quality.