蕨麻素对化学性肝损伤保护作用机制的研究

目的　研究蕨麻素对小鼠化学性肝损伤的保护作用的机制。方法　采用四氯化碳 (CCl4)、半乳糖胺 (D GalN)和对乙酰氨基酚 (AAP)所致小鼠化学性肝损伤模型进行研究 ,分别采用TBA法、DTNB法、CHOD PAP法和IFCC法测定丙二醛 (MDA)、谷胱甘肽 (GSH PX)、甘油三酯 (TG)和碱性磷酸酶 (ALP)。结果　蕨麻素具有促进肝脏糖原合成和提高血清蛋白含量的作用(P <0 .0 1) ,可明显降低肝匀浆脂质过氧化产物MDA的含量 (P <0 .0 1)及提高血清和肝脏过氧化物酶活力 (P <0 .0 1) ,可使AAP致肝损伤小鼠的ALP活性显著下降 ,并降低血清中TG含量。结论　蕨麻素可能通过影响肝脏代谢机能 ,增强抗氧化作用 ,加强解毒能力机制发挥抗损伤作用。

Study on Protective Mechanism of JMS on Chemical Liver Injury

Aim To study the mechanism of JMS protective effects on experimental hepatic injury in mice.Methods Using models of hepatic injury induced by hepatoxicity CCl 4,D-GalN and AAP,together with the technique of TBA,DTNB,CHOD-PAP and IFCC,MDA,GSH-PX,TG and ALP were examined respectively.Results JMS could promote synthesis of hepatic glycogen and improve the level of serum protein.It also could significantly reduce the level of MDA and increase activity of GSH-PX in hepatic homogenate.The level of ALP and TG in serum of liver injured mice induced by AAP was also reduced greatly.Conclusions The mechanism of JMS protective effect on liver-injured mice should involve influencing liver metabolism,improving capability of anti-oxidation and increasing the function of detoxification.