E2F3 及C-myc 在膀胱移行细胞癌组织中的表达及其相关性研究*

目的:探讨E2F3 及C-myc 在膀胱癌组织中表达的相互关系及其与膀胱癌恶性生物学行为之间的关系。方法:应用免疫组织化学方法检测不同病理分期及组织分级的BTCC标本和正常膀胱黏膜中E2F3 及C-myc 蛋白的表达情况，应用免疫组化自动分析系统进行分析，结果以积分光密度表示。结果:膀胱移行细胞癌组织中E2F3 及C-myc 蛋白的表达阳性率明显高于正常膀胱黏膜，两组间有显著性差异（P<0.05），同时E2F3 的表达率与膀胱癌的分级和分期密切相关（P<0.05；P<0.01）；C-myc 的表达率与膀胱癌的分级和分期密切相关（P<0.01；P<0.01）。 膀胱移行细胞癌中E2F3 与C-myc 的表达呈正相关。结论:E2F3 及C-myc 与膀胱癌的恶性程度密切相关，同时二者之间的表达密切相关，二者不仅可以作为膀胱癌的诊断与预后指标，而且为以E2F3 为靶点的膀胱癌的基因治疗提供理论依据。 

Expression of E2F3 and C-Myc in Tissue of Bladder Transitional Cell Carcinoma and Its Significance

Objective: To detect the expression of E2F3 and c-Myc proteins in bladder transitional cell carcinoma (BTCC) tissue and normal bladder epithelial tissue and to analyze the correlation of E 2F3 and c-Myc expression with the bi -ological behaviors of BTCC. Methods:Immunohistochemistry was used to detect the expression of E 2F3 and c-Myc in BTCC tissue (n=64) and normal bladder mucosa ( n=10). Immunohistochemical results were analyzed with Image-Pro Plus software and the corresponding results were reported as integrated optical densities (IOD). Results: The expression of E2F3 and c-Myc in BTCC tissue was higher than that in normal bladder mucosa. The expression of E2F3 and c-Myc was correlated with pathological grade and clinical stage (P<0.05, P<0.01; P<0.01, P<0.01). Immunohistochemistry indicated that the IOD of E 2F3 and c-Myc in BTCC tissue was significantly higher than that in normal bladder mucosa ( P<0.01, P< 0.01). E 2F3 expression was correlated with c-Myc expression ( P<0.01). Conclusion:E2F3 and c-Myc protein levels can be used as diagnostic and prognostic indices of BTCC. This study provides a primary theoretical basis for gene target therapy of BTCC.