口腔放线菌对白假丝酵母菌拮抗作用的体外实验研究

目的观测3种口腔放线菌对白假丝酵母菌的生长有无抑制作用。方法采用直接点种法观察抑菌圈，分别采用反点种菌落计数法和液体共培养法，观察在口腔放线菌作用下白假丝酵母菌菌落数量的变化。结果1）各实验组均未观察到清晰的抑菌圈；2）在黏性放线菌软琼脂上的白假丝酵母菌菌落数较对照组明显减少（P<0.05）；在溶牙放线菌和内氏放线菌软琼脂上无明显白假丝酵母菌生长，与对照组相比差异有统计学意义（P<0.01）；黏性放线菌组与溶牙放线菌组、内氏放线菌组相比，白假丝酵母菌生长差异有统计学意义（P<0.01）；3）分别与3种放线菌共培养的白假丝酵母菌菌落数量减少，与对照组相比差异有统计学意义（P<0.05）；3个实验组间差异无统计学意义（P＞0.05）。结论口腔黏性放线菌、溶牙放线菌和内氏放线菌对白假丝酵母菌的体外生长有抑制作用。

Study the inhibitory effects of three oral Actinomyces on growth of oral Candida albicans in vitro

Objective The aim of this study was to investigate whether the three species of oral Actinomyces have inhibitory effects on the growth of oral Candida albicans in vitro. Methods Straight o′ clock method was used to observe the bacteriostasis circle. Reverse o′ clock and mixed culture method were used to study the quantitative changes of Candida albicans colony respectively. Results 1）None of the groups had been viewed the bacteriostasis circle. 2）Compared with control groups, there was a significant decrease of Candida albicans colony on Actinomyces viscosus TPY soft agar（P <0.05）. Actinomyces naeslundii and Actinomyces odontolyticus TPY soft agar were both devoid of obvious Candida albicans colony（P <0.01）. The former group（Actinomyces viscosus） and the two latter groups（Actinomyces naeslundii and Actinomyces odontolyticus） showed a striking contrast（P<0.01）. 3）Compared with control groups, a decrease of Candida albicans showed up in the mixed culture, and the difference was significant（P< 0.05）. The discrepancies among the three experimental groups were of no statistical value（P＞0.05）. Conclusion Oral Actinomyces viscosus, Actinomyces naeslundii and Actinomyces odontolyticus could inhibit the growth of Candida albicans in vitro. However, which of them contributed more to the inhibitory effects was still not affirmed.