北五味子多糖对脂多糖诱导中性粒细胞炎症的抑制作用

目的：探讨满药北五味子多糖（NSCP）对脂多糖（LPS）激活的离体培养人中性粒细胞的影响，阐明其抗炎作用机制。方法：利用LPS处理人中性粒细胞建立炎症细胞模型，实验分为对照组、LPS组（1.0 mg·L^-1）及NSCP组（1.25、2.50和5.00 g·L^-1），NSCP组先加入LPS刺激60 min，再加入不同浓度NSCP。应用酶联免疫吸附法（ELISA）检测中性粒细胞中肿瘤坏死因子α（TNF-α）水平，采用流式细胞术检测中性粒细胞凋亡率。结果：与对照组比较，LPS组TNF-α水平升高（P < 0.05）；与LPS组比较，NSCP组TNF-α水平降低（P < 0.05）。LPS组中性粒细胞凋亡百分率较对照组降低（P < 0.05）；NSCP组中性粒细胞凋亡百分率随时间及剂量增加而升高，其中5.00 g·L^-1NSCP作用16 h促进其凋亡作用最佳，与LPS组比较凋亡百分率明显升高（P < 0.05）。结论：NSCP可通过抑制LPS诱导TNF-α分泌及中性粒细胞凋亡延迟而发挥抗炎作用。

Inhibitory effect of north-Schisandra chinensis polysaccharide on neutrophil inflammation induced by lipopolysaccharide

Objective:To investigate the effect of Manchu medicine north-Schisandra chinensis polysaccharide (NSCP) on the human neutrophils treated by lipopolysaccharide (LPS) cultivated in vitro,and to elucidate its anti inflammatory mechanism.Methods:The neutrophilic inflammatory cell model was established with LPS.The experiment included control group,LPS group (1.0 mg · L-1) and NSCP group (1.25,2.50 and 5.00 g · L-1),the cells in NSCP group were first treated with LPS for 60 min,and then treated with different concentrations of NSCP.The levels of TNF-α in neutrophils were measured with ELISA and the apoptotic rates were detected by flow cytometry.Results:The level of TNF-a in LPS group was increased compared with control group (P＜0.05).The level of TNF-α in NSCP group was decreased compared with LPS group (P＜0.05).The apoptotic rate in LPS group was decreased compared with control group (P＜0.05);the apoptotic rates in NSCP group were increased with the increasing of time and dose,and the best effect was found 16 h after treatment with 5 g · L-1NSCP:the apoptotic rate in NSCP group was significantly increased compared with LPS group (P＜0.05).Conclusion:NSCP can perform the anti-inflammation effect through the suppression of LPS-induced TNF-α secretion in neutrophils and the promotion of neutrophils apoptosis.