东北鹤虱多糖对免疫抑制小鼠腹腔巨噬细胞功能的影响

目的：研究东北鹤虱多糖（LEGPS-Ⅱ）对免疫抑制小鼠腹腔巨噬细胞（MФ）功能的影响。方法：采用免疫力低下小鼠，通过中性红实验，观察LEGPS-Ⅱ对小鼠静止和ConA活化的腹腔M中体外吞噬功能的影响；采用噻唑蓝比色法（MTT），观察LEG—PS-Ⅱ对小鼠静止和ConA活化的腹腔M中体外分泌IL—1β与TNF—α的影响。结果：MФ体外吞噬实验表明，LEGPS—Ⅱ在0．0156-0．25μg·mL^-1的浓度范围内可剂量依赖性地增加静止和ConA活化的免疫力低下小鼠腹腔MФ吞噬中性红的能力；LEGPS—Ⅱ在20—80μg·mL^-1的浓度范围内可剂量依赖性地增加ConA活化的免疫低下小鼠腹腔MФ分泌IL-1β，但不能有效刺激免疫低下小鼠静止的腹腔MФ产生IL-1β；LEGPS-Ⅱ在10～80μg·mL^-1。的浓度范围内可增加静止和ConA活化的免疫力低下小鼠腹腔MФ分泌TNF—α，且刺激作用具有剂量依赖性。结论：LEGPS-Ⅱ可以通过提高小鼠腹腔MФ的吞噬能力，并增加IL-1β与TNF—α．的分泌而增强MФ的免疫调节功能。

Effects of polysaccharide from Lappula Echinata Gilib on peritoneal macrophage in immunosuppressive micein vitro

Objective:To study the immunoregulatory effects of polysaccharide from Lappula Echinata Gilib(LEGPS-Ⅱ) on immunosuppressive murine peritoned macrophage in vitro.Methods: The effects of LEGPS-Ⅱ on phagocytosis of resting and ConA-activated macrophage in immunosuppressive mice were detected with the neutral red method in vitro.The effects of LEGPS-Ⅱ on the cytokine about IL-1β and TNF-α from resting and ConA-activated peritoned macrophage in immunosuppressive mice were assayed by MTT method in vitro.Results: In vitro,the macrophage phagocytosis experiments showed that LEGPS-Ⅱ(0.015 6～0.25 μg·mL-1) had significant enhancement on phagocytosis of resting and ConA-activated peritoned macrophage with the dose-dependent effect.LEGPS-Ⅱ(20～80 μg·mL-1) could increase the level of IL-1β secreted by ConA-activated macrophage in immunosuppressive mice,but the level could not be increased in the resting macrophage.LEGPS-Ⅱ(10～80 μg·mL-1) had obvious enhancement on the TNF-α secretion of resting and ConA-activated peritoneal macrophage in immunosuppressive mice with the dose-dependent tendency.Conclusion: These indicate that LEGPS-Ⅱ expresses its specific and nonspecific immunoregulatory effects by improving the phagocytosis and enhancing the secretion of TNF-α and IL-1β in macrophage in vitro.