Development and validation of a method for the quantitation of Delta9 tetrahydrocannabinol in human plasma by high performance liquid chromatography after solid-phase extraction |
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| Authors: | Abbara Chadi Galy Romain Benyamina Amine Reynaud Michel Bonhomme-Faivre Laurence |
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| Affiliation: | 1. Division of Neurology, Department of Medicine, Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand;2. Bumrungrad International Hospital, Bangkok 10110, Thailand;3. Department of Neurology, Prasat Neurological Institute, Bangkok 10400, Thailand;4. Department of Neurology, Tohoku University School of Medicine, Sendai 980-8574, Japan;5. Department of Neurology, Yonezawa National Hospital, Yonezawa 992-1202, Japan;6. Department of Multiple Sclerosis Therapeutics, Tohoku University Graduate School of Medicine, Sendai 980-8574, Japan |
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| Abstract: | A high performance liquid chromatography (HPLC) procedure for the determination of Delta9 tetrahydrocannabinol (THC) in human plasma is described. A two-step solid-phase extraction on CN cartridges was coupled with a reversed phase HPLC system. THC was eluted using a mobile phase composed of methanol, acetonitrile and tetrabutylammonium perchlorate solution (0.005 M, pH 3.2), through a C18 Nucleosil column and detected at a wavelength of 215 nm. Calibration curve was linear over the range 5-100 ng/ml with a lower limit of quantification validated at 5 ng/ml. Extraction recovery using the developed extraction procedure was higher than 85%. This method is presently used for the quantification of THC in plasma samples from regular cannabis smokers. |
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