| 应用荧光原位杂交技术对50例羊水标本产前诊断的研究 |
| |
| 引用本文： | 汪广杰,张晓莉,罗阳,陈庆海,府伟灵.应用荧光原位杂交技术对50例羊水标本产前诊断的研究[J].第三军医大学学报,2009,31(8). |
| |
| 作者姓名： | 汪广杰 张晓莉 罗阳 陈庆海 府伟灵 |
| |
| 作者单位： | 第三军医大学西南医院检验科,重庆,400038 |
| |
| 摘 要： | 目的 探讨荧光原位杂交技术诊断未培养羊水细胞非整倍体的临床应用价值.方法 选用X、Y、13、18、2l号特异性探针对50例有产前诊断指征的孕妇进行羊水间期细胞的FISH分析及染色体核型分析.结果 被检50例羊水未培养细胞均获得诊断结果,检测出"21 三体"3例,检测结果与染色体核型分析及随访相符.结论 应用染色体特异性探针对羊水间期细胞进行FISH分析可用于胎儿染色体非整倍体的产前诊断,该方法具有快速、准确、灵敏度高的优点.
|
| 关 键 词： | 荧光原位杂交 羊水间期细胞 产前诊断 |
Prenatal diagnosis for chromosome aneuploidies in 50 amniotic fluid samples with fluorescence in situ hybridization |
| |
| WANG Guang-jie,ZHANG Xiao-li,LUO Yang,CHEN Qing-hai,FU Wei-ling.Prenatal diagnosis for chromosome aneuploidies in 50 amniotic fluid samples with fluorescence in situ hybridization[J].Acta Academiae Medicinae Militaris Tertiae,2009,31(8). |
| |
| Authors: | WANG Guang-jie ZHANG Xiao-li LUO Yang CHEN Qing-hai FU Wei-ling |
| |
| Institution: | WANG Guang-jie,ZHANG Xiao-li,LUO Yang,CHEN Qing-hai,FU Wei-ling(Department of Laboratory Medicine,Southwest Hospital,Third Military Medical University,Chongqing 400038,China) |
| |
| Abstract: | Objective To evaluate the feasibility of using fluorescence in situ hybridization for the detection of a few common chromosome aneuploidies on interphase nuclei of uncultured amniotic fluid cells.Methods A total of 50 patients with indications of chromosome aneuploidies underwent amniocentesis or cordocentesis with karyotyping.Simultaneously,interphase FISH with X,Y,13,18 and 21 chromosome-specific probes was done on them.Results Each of the 50 uncultured amniotic fluid samples tested with FISH was enumerat... |
| |
| Keywords: | fluorescence in situ hybridization interphase amniocyte prenatal diagnosis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
