吗啡依赖及戒断小鼠脑内节律基因mPeriod1表达的研究

目的研究吗啡依赖和吗啡戒断小鼠脑组织内节律基因mPeriod1(mPer1)表达的变化。方法以雄性BALB/C小鼠的吗啡条件性位置偏爱为指标,观测吗啡依赖组、吗啡戒断组小鼠与正常盐水组小鼠的药物精神依赖的变化;应用免疫组化SP法检测3组小鼠脑内mPer1的表达情况;利用图像软件对免疫组化结果进行分析和处理。结果吗啡依赖组的小鼠脑内mPer1表达峰值相位较正常盐水组有明显改变。而吗啡戒断组小鼠脑内mPer1表达峰值相位较正常盐水组没有显著性差异。结论研究结果表明吗啡成瘾影响了以mPer1为重要部件的近日钟的自激振荡过程,使近日节律发生了改变。

Study on Expression of Circadian Gene mPeriod1 (mPer1) in Mice Brain of Morphine Dependence and Withdrawal

Objective To investigate the variation of the circadian gene mPeriod1 (mPer1) expression in the brain of mice treated with morphine and after stopping treatment. Method Conditioned place preferences of mice treated with morphine and after stopping treated mice were observed. The expression of mPer1 in the brain of the mice was investigated by immunohistochemistry. Then, the immunohistochemical results were analyzed by the image analyzing software. Result The phase of the peak of mPer1 in morphine treated mice was obviously changed as compared with the control mice, but no significant difference was found between the control and the mice after stopping morphine treatment. Conclusion The self-oscillation of circadian gene mPer1 is affected by morphine treatment and the circadian rhythm of mice is disturbed.