Comparison of the plaque assay and 50% tissue culture infectious dose assay as methods for measuring filovirus infectivity |
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| Authors: | Sophie J. Smither Calli Lear-Rooney Julia Biggins Jamie Pettitt Mark S. Lever Gene G. Olinger Jr. |
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| Affiliation: | 1. Biomedical Sciences Department, Defence Science and Technology Laboratory (DSTL), Porton Down, Salisbury, Wiltshire SP4 0JQ, UK;2. Virology Division, U.S. Army Medical Research Institute of Infectious Diseases (USAMRIID), 1425 Porter Street, Frederick, MD 21701, USA |
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| Abstract: | Two common methods of quantifying filovirus infectivity, a plaque assay and 50% cell culture infectious dose (TCID50) endpoint dilution assay, were compared. The two assays were performed side by side using the same virus stock sample to determine the correlation between the results of the two assays. The TCID50 assay appeared to be more sensitive but slightly more variable, and there was a tenfold difference in the numerical results of these methods of enumeration. The advantages and disadvantages of both assays are discussed. Both methods are useful and practicable in filovirus research, and this comparison will be hugely beneficial to the filovirus research community as it seeks to become more united. Further work in this area should be performed to ensure consistency in filovirus research. |
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| Keywords: | ACDP, Advisory Committee for Dangerous Pathogens BSL, biosafety level CPE, cytopathic effects PFU, plaque forming unit PD, proportional distance TCID50, 50% tissue culture infectious dose FBS, fetal bovine serum FCS, fetal calf serum |
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