高危人群生殖支原体分离培养的初步报告

目的　用病原学方法了解我国高危人群是否有生殖支原体感染及其频率 ,为防治工作提供依据。方法　取性病患者及性罪错者泌尿生殖道分泌物 ,接种在SP 4培养基作分离培养 ,符合生殖支原体生物学特征的培养物用聚合酶链反应技术加以鉴定。结果　在 2 2 7例高危人群中培养出 8株生殖支原体 ,总分离率为 3 85 %。其中性病患者分离率为 5 2 1 %,性罪错者为 2 2 9%。两者差异无显著性 (P >0 0 5 )。结论　从病原学上首次证实 ,京、沪、宁三地高危人群中存在生殖支原体感染 ,在性传播疾病防治工作中应予重视

Preliminary Study on the Isolation of Mycoplasma genitalium in the High Risk Populations in China

Objective The purpose of this study is to understand whether Mycoplasma genitalium infection is present in the high risk populations in China, and what is its prevalence so as to provide evidence for the prevention and treatment of it. Methods The genitourinary specimens collected from the STD patients and sexually promiscuous persons were cultured in SP 4 medium for the isolation of M.genitalium. All of the isolates with their biological characteristics consistent with those of M.genitalium were identified by polymerase chain reaction(PCR). Results Eight strains of M.genitalium were isolated from 227 genitourinary specimens, the isolation rate of M.genitalium in the high risk populations was 3 85%, among them the rates in the STD patients and in the promiscuous persons were 5 21% and 2 29% respectively,and the difference between them was not significant (P>0 05). Conclusion M.genitalium infection is present in the high risk populations in Beijing,Shanghai and Nanjing, and is firstly verified by isolation of pathogen.