1例阴沟肠杆菌碳青霉烯类抗生素耐药的机制研究

目的 对浙江省瑞安市人民医院临床分离的1株碳青霉烯类抗生素耐药的阴沟肠杆菌的耐药机制进行研究。 方法 通过E-test检测阴沟肠杆菌及接合菌株的最低抑菌浓度,Hodge试验检测碳青霉烯酶,采用接合实验、聚合酶链反应(PCR)、 质粒抽提、琼脂糖凝胶电泳及耐药基因克隆测序分析细菌的耐药机制。 结果 接合试验显示阴沟肠杆菌中的碳青霉烯类耐药基因位于质粒上,基因扩增、测序、比对等方法测得此耐药基因为BlaKPC-2型。 结论 阴沟肠杆菌碳青霉烯类药物耐药由质粒介导的KPC-2酶引起。

Carbopenems resistant mechanism of a strain of Enterobacter cloacae

Objective To understand the carbopenems resistant mechanism of a strain of Enterobacter cloacae isolated in our hospital.Methods The minimal inhibitory concentrations of the Enterobacter cloacae strain and conjugate bacterium were detected with E-test,the carbapenemase was detected with Hodge test.Conjugation test,polymerase chain reaction(PCR),plasmid extraction,agarose gel electrophoresis and cloning and sequencing of drug resistant gene were conducted to analyze the resistant mechanism.Results Conjugatio...