沙门菌属LAMP检测方法在食品卫生检验中的应用评价

目的探讨沙门菌属环介导等温扩增(loop-mediated isothermal amplification,LAMP)方法在食品卫生检验中的应用价值。方法利用文献报道的LAMP检测方法和分离培养法分别对135份食品标本进行沙门菌检测,评价LAMP法和分离培养法的一致性。结果 LAMP方法检出14份食品标本沙门菌阳性,阳性率为10.4%,电泳和加荧光染料染色后均能判断结果;分离培养法检出6份食品标本沙门菌阳性,阳性率为4.4%。LAMP法检出阳性率高于培养法(P=0.021)。LAMP方法可在24 h内从食品中检测出沙门菌,而分离培养法检出沙门菌需要4~7 d,LAMP检测方法更快速。结论沙门菌属LAMP方法检测阳性率高于分离培养法,LAMP方法可用于食品中沙门菌的核酸检测。

Evaluation and application of loop-mediated isothermal amplification assay for salmonella in food sanitation analysis

Objective To evaluate the application value of loop-mediated isothermal amplification （LAMP） for salmonella in food sanitation analysis. Methods 135 specimens of food were detected with the LAMP assay and isolated culture assay for salmonella from the other study. The consistency of the LAMP assay and isolated culture assay were com- pared. Results After LAMP reaction, the positive results were all observed with electrophoresis and SYBR~ Green I nu- cleic gel stain （SY]3R Green I） staining in 14 specimens of food, the positive rate was 10.4%. 6 positive specimens were detected in the isolated culture assay, the positive rate was 4.4%. The results of the two methods had significant differences, the positive rate of LAMP method was higher than the isolated culture assay （P = 0. 021 ）. LAMP was superior to isolated culture a~ay in terms of rapidity, because salmonella in food were detected with the LAMP assay within 24 hours, but isolated culture assay needed 4 to 7 days. Conclusions The positive rate of LAMP method is higher than the isolated culture assay for salmonella, the LAMP method can be used to nucleotide detection of salmonella in food.