| 应用VNTR技术对绵阳地区结核分枝杆菌进行基因分型 |
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| 引用本文： | 陈志,陈佳,李六林,先德强,岳蕴瑶,江智辉,周良君.应用VNTR技术对绵阳地区结核分枝杆菌进行基因分型[J].疾病控制杂志,2011,15(9):810-813. |
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| 作者姓名： | 陈志 陈佳 李六林 先德强 岳蕴瑶 江智辉 周良君 |
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| 作者单位： | 1. 绵阳市疾病预防控制中心理化检验科,四川,绵阳,621000
2. 绵阳市中心医院儿科,四川,绵阳,621000 |
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| 基金项目： | 四川省卫生厅课题资助项目(080213); 绵阳市卫生局课题资助项目(200703) |
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| 摘 要： | 目的应用数目可变串联重复序列(variable number of tandem repeats,VNTRs)分析技术探讨绵阳地区结核分枝杆菌的基因分型,为结核病的防治提供科学依据。方法选取绵阳地区结核分枝杆菌临床分离菌株,采用聚合酶链反应(PCR)分别对VNTR位点进行检测分析,应用BioNumerics 5.0软件进行聚类分析。结果共对79株结核分枝杆菌的15个VNTR位点进行了检测,结果显示明显的基因多态性。各个位点的分辨能力不同,QUB-11b位点的分辨指数(Hunter-Gaston index,HGI)最高,为0.880;ETR C的HGI最低,为0.234;总HGI达到1。经聚类分析,79株菌可分为7个基因群、79个基因型,以Ⅲ群和Ⅴ群所占比例较大,Ⅲ群含37株菌,占46.8%;Ⅴ群含33株菌,占41.8%,其他菌株呈散在分布。结论绵阳地区结核分枝杆菌VNTRs基因存在明显的多态性,主要流行菌群为Ⅲ群和Ⅴ群,应加强此两群菌株的监控。
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| 关 键 词： | 分枝杆菌 结核 基因分型 串联重复序列 |
Genotyping of mycobacterium tuberculosis with VNTR technique in Mianyang,Sichuan |
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| CHEN Zhi,CHEN Jia,LI Liu-lin,XIAN De-qiang,YUE Yun-yao,JIANG Zhi-hui,ZHOU Liang-jun.Genotyping of mycobacterium tuberculosis with VNTR technique in Mianyang,Sichuan[J].Chinese Journal of Disease Control and Prevention,2011,15(9):810-813. |
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| Authors: | CHEN Zhi CHEN Jia LI Liu-lin XIAN De-qiang YUE Yun-yao JIANG Zhi-hui ZHOU Liang-jun |
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| Institution: | CHEN Zhi1,CHEN Jia2,LI Liu-lin1,XIAN De-qiang1,YUE Yun-yao1,JIANG Zhi-hui1,ZHOU Liang-jun1.1.Department of Physico-Chemical Laboratory,Mianyang Center for Disease Control and Prevention,Mianyang 621000,China,2.Department of Pediatrics,Central Hospital of Mianyang City |
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| Abstract: | Objective To discuss the genotyping of mycobacterium tuberculosis clinical isolates in Mianyang area by variable number tandem repeats(VNTRs) technique and provide scientific basis for the prevention and control of tuberculosis.Methods Mycobacterium tuberculosis clinical isolates were selected from Mianyang area,the polymorphism of VNTR locus was tested with PCR.The clustering of genotype was analyzed with BioNumerics 5.0.Results 15 VNTR loci of 79 mycobacterium tuberculosis clinical isolates were analyzed ... |
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| Keywords: | Mycobacterium tuberculosis Genotyping Tandem repeat sequences |
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